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Comparative Study
. 1991 Jul;173(14):4474-81.
doi: 10.1128/jb.173.14.4474-4481.1991.

Growth phase-regulated expression of bolA and morphology of stationary-phase Escherichia coli cells are controlled by the novel sigma factor sigma S

R Lange  1 , R Hengge-Aronis
Affiliations
Comparative Study

Growth phase-regulated expression of bolA and morphology of stationary-phase Escherichia coli cells are controlled by the novel sigma factor sigma S

R Lange et al. J Bacteriol. 1991 Jul.

Abstract

The novel sigma factor (sigma S) encoded by rpoS (katF) is required for induction of many growth phase-regulated genes and expression of a variety of stationary-phase phenotypes in Escherichia coli. Here we demonstrate that wild-type cells exhibit spherical morphology in stationary phase, whereas rpoS mutant cells remain rod shaped and are generally larger. Size reduction of E. coli cells along the growth curve is a continuous and at least biphasic process, the second phase of which is absent in rpoS-deficient cells and correlates with induction of the morphogene bolA in wild-type cells. Stationary-phase induction of bolA is dependent on sigma S. The "gearbox" a characteristic sequence motif present in the sigma S-dependent growth phase- and growth rate-regulated bolAp1 promoter, is not recognized by sigma S, since stationary-phase induction of the mcbA promoter, which also contains a gearbox, does not require sigma S, and other sigma S-controlled promoters do not contain gearboxes. However, good homology to the potential -35 and -10 consensus sequences for sigma S regulation is found in the bolAp1 promoter.

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References

    1. J Bacteriol. 1990 Dec;172(12):6697-703 - PubMed
    1. J Bacteriol. 1973 Jun;114(3):1068-73 - PubMed
    1. Mol Microbiol. 1991 Jan;5(1):49-59 - PubMed
    1. J Bacteriol. 1991 Jul;173(14):4482-92 - PubMed
    1. J Bacteriol. 1991 Jan;173(2):514-20 - PubMed

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