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. 2006 Feb 14:6:5.
doi: 10.1186/1471-213X-6-5.

Zebrafish transgenic Enhancer TRAP line database (ZETRAP)

Benjamin G H Choo  1 , Igor Kondrichin, Sergey Parinov, Alexander Emelyanov, William Go, Wei-chang Toh, Vladimir Korzh
Affiliations

Zebrafish transgenic Enhancer TRAP line database (ZETRAP)

Benjamin G H Choo et al. BMC Dev Biol. .

Abstract

Background: The zebrafish, Danio rerio, is used as a model organism to study vertebrate genetics and development. An effective enhancer trap (ET) in zebrafish using the Tol2 transposon has been demonstrated. This approach could be used to study embryogenesis of a vertebrate species in real time and with high resolution.

Description: The information gathered during the course of systematic investigation of many ET transgenic lines have been collected and compiled in the form of an online database--the Zebrafish Enhancer TRAP lines database (ZETRAP).

Conclusion: ZETRAP is a web-based system that provides data and information to the scientific community about the developmental, genetic and genomic aspects of transgenic zebrafish lines obtained using Tol2 transposon-mediated transgenesis. The current version (version 1.0) contains description of 27 ET lines that express EGFP in various organs and tissues, for example, heart, brain, notochord, gut, etc. It also includes information on insertion sites of the Tol2 transposon in these lines.

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Figures

Figure 1
Figure 1
ZETRAP homepage is kept simple for easy navigation.
Figure 2
Figure 2
The Gene Expression view lists all ET lines and provides their brief description.
Figure 3
Figure 3
ET line view provides a low resolution image of expression pattern, brief description of expression pattern and insertion site.
Figure 4
Figure 4
ET line view also contains several high resolution images and ends by showing TAIL-PCR data of sequences flanking the insertion sites.
Figure 5
Figure 5
The Glossary view provides a list of anatomical terms used. These are hyperlinked to the ET lines containing GFP expression in these tissues and organs.
See this image and copyright information in PMC

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