Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jan;15(1):161-6.
doi: 10.1128/JVI.15.1.161-166.1975.

Molecular weight of RNA subunits of Rous sarcoma virus determined by electron microscopy

A B Jacobson, P A Bromley

Molecular weight of RNA subunits of Rous sarcoma virus determined by electron microscopy

A B Jacobson et al. J Virol. 1975 Jan.

Abstract

Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 muCi of [32P]orthophosphate per ml. Virus was collected by centrifugation, and the RNA was extracted and denatured with dimethyl sulfoxide, and the 33S subunit RNA was isolated by sucrose gradient centrifugation. The molecular weight of the RSV subunit RNA was determined by length measurement in the electron microscope, by using bacteriophage MS2 RNA as a length marker. Molecules of between 2.5 and 3.3 mum in length made up over 50% of the subunit RNA preparation. In this paper, we define RSV RNA subunits as that RNA released from the 70S RNA complex by dimethyl sulfoxide treatment, which sediments as a peak at 33S. Assuming the molecular weight of MS2 RNA to be 1.2 times 10-6, we calculate the molecular weight of RSV subunit RNA to be 3.12 times 10-6 plus or minus 0.25 times 10-6.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1964 Apr;8:496-507 - PubMed
    1. J Gen Virol. 1969 Apr;4(3):449-52 - PubMed
    1. J Mol Biol. 1966 Apr;16(2):571-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511-8 - PubMed
    1. Virology. 1969 Jan;37(1):124-31 - PubMed

LinkOut - more resources