doi: 10.1016/j.cell.2005.10.020.
Epub 2005 Nov 3.
Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation
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- PMID: 16271385
- DOI: 10.1016/j.cell.2005.10.020
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Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation
Cell.
.
Free article
Abstract
The mRNA-cleavage step of RNA interference is mediated by an endonuclease, Argonaute2 (Ago2), within the RNA-induced silencing complex (RISC). Ago2 uses one strand of the small interfering (si) RNA duplex as a guide to find messenger RNAs containing complementary sequences and cleaves the phosphodiester backbone at a specific site measured from the guide strand's 5' end. Here, we show that both strands of siRNA get loaded onto Ago2 protein in Drosophila S2 cell extracts. The anti-guide strand behaves as a RISC substrate and is cleaved by Ago2. This cleavage event is important for the removal of the anti-guide strand from Ago2 protein and activation of RISC.
Comment in
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RNAi: RISC gets loaded.Cell. 2005 Nov 18;123(4):543-5. doi: 10.1016/j.cell.2005.11.006. Cell. 2005. PMID: 16286001
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