Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin
- PMID: 16061184
- DOI: 10.1016/j.molcel.2005.06.011
Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin
Abstract
Methylation of histones modulates chromatin structure and function. Whereas methylation of histone H3 on lysines 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9 and 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes within euchromatin. Here, we show that H3K9 di- and trimethylation occur in the transcribed region of active genes in mammalian chromatin. This modification is dynamic, as it increases during activation of transcription and is rapidly removed upon gene repression. Heterochromatin Protein 1gamma (HP1gamma), a protein containing a chromo-domain that recognizes H3K9 methylation, is also present in the transcribed region of all active genes examined. Both the presence of HP1gamma and H3K9 methylation are dependent upon elongation by RNA polymerase II. These findings demonstrate novel roles for H3K9 methylation and HP1gamma in transcription activation.
Similar articles
-
The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36.Nature. 2006 Jul 20;442(7100):312-6. doi: 10.1038/nature04853. Epub 2006 May 28. Nature. 2006. PMID: 16732292
-
Histone lysine trimethylation exhibits a distinct perinuclear distribution in Plzf-expressing spermatogonia.Dev Biol. 2006 May 15;293(2):461-72. doi: 10.1016/j.ydbio.2006.02.013. Epub 2006 Mar 20. Dev Biol. 2006. PMID: 16549060
-
Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair.Biochemistry. 2006 Mar 7;45(9):2852-61. doi: 10.1021/bi0521083. Biochemistry. 2006. PMID: 16503640
-
Leaving a mark: the many footprints of the elongating RNA polymerase II.Curr Opin Genet Dev. 2006 Apr;16(2):184-90. doi: 10.1016/j.gde.2006.02.004. Epub 2006 Feb 28. Curr Opin Genet Dev. 2006. PMID: 16503129 Review.
-
A methylation rendezvous: reader meets writers.Dev Cell. 2007 Jun;12(6):843-4. doi: 10.1016/j.devcel.2007.05.011. Dev Cell. 2007. PMID: 17543855 Review.
Cited by
-
Altered Transcriptional Control Networks with Trans-Differentiation of Isogenic Mutant-KRas NSCLC Models.Front Oncol. 2014 Dec 8;4:344. doi: 10.3389/fonc.2014.00344. eCollection 2014. Front Oncol. 2014. PMID: 25538889 Free PMC article.
-
Recognition of H3K9 methylation by GLP is required for efficient establishment of H3K9 methylation, rapid target gene repression, and mouse viability.Genes Dev. 2015 Feb 15;29(4):379-93. doi: 10.1101/gad.254425.114. Epub 2015 Jan 30. Genes Dev. 2015. PMID: 25637356 Free PMC article.
-
Analysis of transcriptional regulation of the human miR-17-92 cluster; evidence for involvement of Pim-1.Int J Mol Sci. 2013 Jun 7;14(6):12273-96. doi: 10.3390/ijms140612273. Int J Mol Sci. 2013. PMID: 23749113 Free PMC article.
-
A hypomorphic Cbx3 allele causes prenatal growth restriction and perinatal energy homeostasis defects.J Biosci. 2015 Jun;40(2):325-38. doi: 10.1007/s12038-015-9520-x. J Biosci. 2015. PMID: 25963260
-
CBX3 regulates efficient RNA processing genome-wide.Genome Res. 2012 Aug;22(8):1426-36. doi: 10.1101/gr.124818.111. Epub 2012 Jun 8. Genome Res. 2012. PMID: 22684280 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
