Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2005;26(2-3):113-22.
doi: 10.1385/JMN:26:2-3:113.

Entropy and oligomerization in GPCRs

Rajkumar P Thummer  1 , Matthew P Campbell, Mark K Dean, Marie J Frusher, Paul D Scott, Christopher A Reynolds
Affiliations
Review

Entropy and oligomerization in GPCRs

Rajkumar P Thummer et al. J Mol Neurosci. 2005.

Abstract

Evolutionary trace (ET) and entropy are two related methods for analyzing a multiple sequence alignment to determine functionally important residues in proteins. In this article, these methods have been enhanced with a view to reinvestigate the issue ofGPCR dimerization and oligomerization. In particular, cluster analysis has replaced the subjective visual analysis element of the original ET method. Previous applications of the ET method predicted two dimerization interfaces on the external transmembrane lipid-facing region of GPCRs; these were discussed in terms of dimerization and linear oligomers. Removing the subjective element of the ET method gives rise to the prediction of functionally important residues on the external face of each transmembrane helix for a large number of class A GPCRs. These results are consistent with a growing body of experimental information that, taken over many receptor subtypes, has implicated each transmembrane helix in dimeric interactions. In this application, entropy gave superior results to those obtained from the ET method in that its use gives rise to higher z-scores and fewer instances of z-scores below 3.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Biol Chem. 2002 Sep 20;277(38):34666-73 - PubMed
    1. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7507-11 - PubMed
    1. Biochemistry. 2002 Apr 30;41(17):5588-604 - PubMed
    1. J Biol Chem. 2001 Jun 22;276(25):22621-9 - PubMed
    1. Mol Endocrinol. 2003 Apr;17(4):677-91 - PubMed

Publication types

Substances

LinkOut - more resources