Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1
- PMID: 16009131
- DOI: 10.1016/j.cell.2005.05.008
Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1
Abstract
Estrogen plays an essential physiologic role in reproduction and a pathologic one in breast cancer. The completion of the human genome has allowed the identification of the expressed regions of protein-coding genes; however, little is known concerning the organization of their cis-regulatory elements. We have mapped the association of the estrogen receptor (ER) with the complete nonrepetitive sequence of human chromosomes 21 and 22 by combining chromatin immunoprecipitation (ChIP) with tiled microarrays. ER binds selectively to a limited number of sites, the majority of which are distant from the transcription start sites of regulated genes. The unbiased sequence interrogation of the genuine chromatin binding sites suggests that direct ER binding requires the presence of Forkhead factor binding in close proximity. Furthermore, knockdown of FoxA1 expression blocks the association of ER with chromatin and estrogen-induced gene expression demonstrating the necessity of FoxA1 in mediating an estrogen response in breast cancer cells.
Similar articles
-
From the Cover: Location analysis of estrogen receptor alpha target promoters reveals that FOXA1 defines a domain of the estrogen response.Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11651-6. doi: 10.1073/pnas.0505575102. Epub 2005 Aug 8. Proc Natl Acad Sci U S A. 2005. PMID: 16087863 Free PMC article.
-
ER regulates an evolutionarily conserved apoptosis pathway.Biochem Biophys Res Commun. 2010 Sep 10;400(1):34-8. doi: 10.1016/j.bbrc.2010.07.132. Epub 2010 Aug 4. Biochem Biophys Res Commun. 2010. PMID: 20691160
-
ARID1A determines luminal identity and therapeutic response in estrogen-receptor-positive breast cancer.Nat Genet. 2020 Feb;52(2):198-207. doi: 10.1038/s41588-019-0554-0. Epub 2020 Jan 13. Nat Genet. 2020. PMID: 31932695 Free PMC article.
-
Estrogen receptor target gene: an evolving concept.Mol Endocrinol. 2006 Aug;20(8):1707-14. doi: 10.1210/me.2005-0334. Epub 2006 Jan 5. Mol Endocrinol. 2006. PMID: 16396959 Review.
-
Genomic location analysis by ChIP-Seq.J Cell Biochem. 2009 May 1;107(1):11-8. doi: 10.1002/jcb.22077. J Cell Biochem. 2009. PMID: 19173299 Free PMC article. Review.
Cited by
-
ChIP-exo signal associated with DNA-binding motifs provides insight into the genomic binding of the glucocorticoid receptor and cooperating transcription factors.Genome Res. 2015 Jun;25(6):825-35. doi: 10.1101/gr.185157.114. Epub 2015 Feb 26. Genome Res. 2015. PMID: 25720775 Free PMC article.
-
To bind or not to bind--FoxA1 determines estrogen receptor action in breast cancer progression.Breast Cancer Res. 2012 Jun 19;14(3):312. doi: 10.1186/bcr3146. Breast Cancer Res. 2012. PMID: 22713214 Free PMC article.
-
FOXA1 and breast cancer risk.Nat Genet. 2012 Nov;44(11):1176-7. doi: 10.1038/ng.2449. Nat Genet. 2012. PMID: 23104063
-
Chromatin immunoprecipitation: advancing analysis of nuclear hormone signaling.J Mol Endocrinol. 2012 Sep 5;49(2):R113-23. doi: 10.1530/JME-12-0016. Print 2012 Oct. J Mol Endocrinol. 2012. PMID: 22872135 Free PMC article. Review.
-
Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics.Genome Res. 2012 Jun;22(6):1015-25. doi: 10.1101/gr.133280.111. Epub 2012 Apr 16. Genome Res. 2012. PMID: 22508765 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
