Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis
- PMID: 15951802
- DOI: 10.1038/nature03817
Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis
Abstract
Gradients of signalling and transcription factors govern many aspects of embryogenesis, highlighting the need for spatiotemporal control of regulatory protein levels. MicroRNAs are phylogenetically conserved small RNAs that regulate the translation of target messenger RNAs, providing a mechanism for protein dose regulation. Here we show that microRNA-1-1 (miR-1-1) and miR-1-2 are specifically expressed in cardiac and skeletal muscle precursor cells. We found that the miR-1 genes are direct transcriptional targets of muscle differentiation regulators including serum response factor, MyoD and Mef2. Correspondingly, excess miR-1 in the developing heart leads to a decreased pool of proliferating ventricular cardiomyocytes. Using a new algorithm for microRNA target identification that incorporates features of RNA structure and target accessibility, we show that Hand2, a transcription factor that promotes ventricular cardiomyocyte expansion, is a target of miR-1. This work suggests that miR-1 genes titrate the effects of critical cardiac regulatory proteins to control the balance between differentiation and proliferation during cardiogenesis.
Comment in
-
Developmental biology: tiny brakes for a growing heart.Nature. 2005 Jul 14;436(7048):181-2. doi: 10.1038/436181a. Nature. 2005. PMID: 16015310 No abstract available.
Similar articles
-
Developmental biology: tiny brakes for a growing heart.Nature. 2005 Jul 14;436(7048):181-2. doi: 10.1038/436181a. Nature. 2005. PMID: 16015310 No abstract available.
-
Target identification of microRNAs expressed highly in human embryonic stem cells.J Cell Biochem. 2009 Apr 15;106(6):1020-30. doi: 10.1002/jcb.22084. J Cell Biochem. 2009. PMID: 19229866
-
MiR-495 and miR-218 regulate the expression of the Onecut transcription factors HNF-6 and OC-2.Biochem Biophys Res Commun. 2010 Jan 1;391(1):293-8. doi: 10.1016/j.bbrc.2009.11.052. Epub 2009 Nov 12. Biochem Biophys Res Commun. 2010. PMID: 19913497
-
MicroRNAs in muscle differentiation: lessons from Drosophila and beyond.Curr Opin Genet Dev. 2006 Oct;16(5):533-9. doi: 10.1016/j.gde.2006.08.010. Epub 2006 Aug 17. Curr Opin Genet Dev. 2006. PMID: 16919443 Review.
-
Basic helix-loop-helix proteins expressed during early embryonic organogenesis.Int Rev Cytol. 2004;236:251-80. doi: 10.1016/S0074-7696(04)36006-7. Int Rev Cytol. 2004. PMID: 15261740 Review.
Cited by
-
MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia.Mol Cell Neurosci. 2016 Sep;75:36-43. doi: 10.1016/j.mcn.2016.06.003. Epub 2016 Jun 21. Mol Cell Neurosci. 2016. PMID: 27346077 Free PMC article.
-
Trbp regulates heart function through microRNA-mediated Sox6 repression.Nat Genet. 2015 Jul;47(7):776-83. doi: 10.1038/ng.3324. Epub 2015 Jun 1. Nat Genet. 2015. PMID: 26029872 Free PMC article.
-
MicroRNA regulation and role in stem cell maintenance, cardiac differentiation and hypertrophy.Curr Mol Med. 2013 Jun;13(5):757-64. doi: 10.2174/1566524011313050007. Curr Mol Med. 2013. PMID: 23642057 Free PMC article. Review.
-
Functional screening identifies miRNAs inducing cardiac regeneration.Nature. 2012 Dec 20;492(7429):376-81. doi: 10.1038/nature11739. Epub 2012 Dec 5. Nature. 2012. PMID: 23222520
-
Impaired exercise tolerance, mitochondrial biogenesis, and muscle fiber maintenance in miR-133a-deficient mice.FASEB J. 2016 Nov;30(11):3745-3758. doi: 10.1096/fj.201600529R. Epub 2016 Jul 25. FASEB J. 2016. PMID: 27458245 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
