Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005;12(1):59-64.
doi: 10.1007/s11373-004-8184-6. Epub 2005 Feb 4.

Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome

Pei-Lan Shao  1 , Po-Ren Hsueh, Luan-Yin Chang, Chun-Yi Lu, Chuan-Liang Kao, Yu-Ping Chiang, Hsiang-Yi Huang, Fu-Yuan Huang, Chin-Yun Lee, Lung-Ji Chang, T-C Wu, Li-Min Huang; SARS Research Group of National Taiwan University College of Medicine; National Taiwan University Hospital
Affiliations

Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome

Pei-Lan Shao et al. J Biomed Sci. 2005.

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Lee N., Hui D., Wu A., Chan P., Cameron P., Joynt G.M., Ahuja A., Yung M.Y., Leung C.B., To K.F., Lui S.F., Szeto C.C., Chung S., Sung J.J.Y. A major outbreak of severe acute respiratory syndrome in Hong Kong. New Engl. J. Med. 2003;348:1986–1994. - PubMed
    1. Poutanen S.M., Low D.E., Henry B., Finkelstein S., Rose D., Green K., Tellier R., Draker R., Adachi D., Ayers M., Chan A.K., Skowronski D.M., Salit I., Simor A.E., Slutsky A.S., Doyle P.W., Krajden M., Petric M., Brunham R.C., McGeer A.J. The National Microbiology Laboratory, Canada and the Canadian Severe Acute Respiratory Syndrome Study Team. Identification of severe acute respiratory syndrome in Canada. New Engl. J. Med. 2003;348:1995–2005. - PubMed
    1. Peiris J.S.M., Chu C.M., Cheng V.C.C., Chan K.S., Hung I.F.N., Poon L.L.M., Law K.I., Tang B.S.F., Hon T.Y.W., Chan C.S., Chan K.H., Ng J.S.C., Zheng B.J., Ng W.L., Lai R.W.M., Guan Y., Yuen K.Y. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study. Lancet. 2003;361:1767–1772. doi: 10.1016/S0140-6736(03)13412-5. - DOI - PMC - PubMed
    1. Hsueh P.R., Hsiao C.H., Yeh S.H., Wang W.K., Chen P.J., Wang J.T., Chang S.C., Kao C.L., Yang P.C. Microbiologic characteristics, serologic responses, and clinical manifestations in severe acute respiratory syndrome, Taiwan. Emerg. Infect. Dis. 2003;9:1163–1167. - PMC - PubMed
    1. World Health Organization, 2003. Summary of probable SARS cases with onset of illness from 1 November 2002 to 31 July 2003. [Online] http: //www.who.int/sars/country/table2003_09_23/en/. Accessed 26 September 2003.

Publication types

MeSH terms

LinkOut - more resources