Environmental surveillance of wild poliovirus circulation in Egypt--balancing between detection sensitivity and workload
- PMID: 15847928
- DOI: 10.1016/j.jviromet.2005.02.002
Environmental surveillance of wild poliovirus circulation in Egypt--balancing between detection sensitivity and workload
Abstract
Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. These results emphasize the need to use intensive laboratory analysis to optimise sample sensitivity in environmental poliovirus surveillance, and the difficulties in reproducing the isolation results by simple re-inoculation of samples containing a mixture of different viruses.
Similar articles
-
Rapid detection of polioviruses in environmental water samples by one-step duplex RT-PCR.Southeast Asian J Trop Med Public Health. 2000 Mar;31(1):47-56. Southeast Asian J Trop Med Public Health. 2000. PMID: 11023064
-
Isolation and characterization of circulating type 1 vaccine-derived poliovirus from sewage and stream waters in Hispaniola.J Infect Dis. 2004 Apr 1;189(7):1168-75. doi: 10.1086/382545. Epub 2004 Mar 16. J Infect Dis. 2004. PMID: 15031784
-
Assessment of cell culture and polymerase chain reaction procedures for the detection of polioviruses in wastewater.Bull World Health Organ. 1999;77(12):973-80. Bull World Health Organ. 1999. PMID: 10680244 Free PMC article.
-
Sporadic amyotrophic lateral sclerosis: a hypothesis of persistent (non-lytic) enteroviral infection.Amyotroph Lateral Scler Other Motor Neuron Disord. 2005 Jun;6(2):77-87. doi: 10.1080/14660820510027026. Amyotroph Lateral Scler Other Motor Neuron Disord. 2005. PMID: 16036430 Review.
-
[Capillary gel electrophoresis in clinical molecular biology].Sheng Li Ke Xue Jin Zhan. 1999 Apr;30(2):159-62. Sheng Li Ke Xue Jin Zhan. 1999. PMID: 12532813 Review. Chinese. No abstract available.
Cited by
-
CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters.Front Environ Sci. 2022 Jul 4;10:10.3389/fenvs.2022.914387. doi: 10.3389/fenvs.2022.914387. Front Environ Sci. 2022. PMID: 35928599 Free PMC article.
-
Rapid and Sensitive Direct Detection and Identification of Poliovirus from Stool and Environmental Surveillance Samples by Use of Nanopore Sequencing.J Clin Microbiol. 2020 Aug 24;58(9):e00920-20. doi: 10.1128/JCM.00920-20. Print 2020 Aug 24. J Clin Microbiol. 2020. PMID: 32611795 Free PMC article.
-
Haiti Poliovirus Environmental Surveillance.Am J Trop Med Hyg. 2019 Dec;101(6):1240-1248. doi: 10.4269/ajtmh.19-0469. Am J Trop Med Hyg. 2019. PMID: 31701857 Free PMC article.
-
Feasibility of the Bag-Mediated Filtration System for Environmental Surveillance of Poliovirus in Kenya.Food Environ Virol. 2020 Mar;12(1):35-47. doi: 10.1007/s12560-019-09412-1. Epub 2019 Nov 2. Food Environ Virol. 2020. PMID: 31679104 Free PMC article.
-
Field Performance of Two Methods for Detection of Poliovirus in Wastewater Samples, Mexico 2016-2017.Food Environ Virol. 2019 Dec;11(4):364-373. doi: 10.1007/s12560-019-09399-9. Epub 2019 Sep 30. Food Environ Virol. 2019. PMID: 31571037 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources