Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Apr;5(4):371-6.
doi: 10.1128/AAC.5.4.371.

Rifampin: inhibition of ribonucleic acid synthesis after potentiation by amphotericin B in Saccharomyces cerevisiae

E Battaner, B V Kumar

Rifampin: inhibition of ribonucleic acid synthesis after potentiation by amphotericin B in Saccharomyces cerevisiae

E Battaner et al. Antimicrob Agents Chemother. 1974 Apr.

Abstract

The inhibition of Saccharomyces cerevisiae growth by amphotericin B and rifampin was studied. Rifampin alone had no effect on growth or macromolecular syntheses. Lethal amounts of amphotericin B produced a late inhibition of ribonucleic acid (RNA) synthesis simultaneous with the arrest of growth and protein synthesis. In contrast, low doses of amphotericin B along with rifampin caused an early arrest of RNA synthesis, followed by a later arrest of growth and protein synthesis. Used with rifampin, amphotericin B thus appears to increase cell permeability for rifampin, which in turn inhibits RNA synthesis; such results are consistent with some reports of inhibition of yeast RNA polymerase function by rifampin. Experiments with petite mutants ruled out any special effect of the antibiotics on mitochondrial RNA synthesis, so that nuclear RNA synthesis is affected. Acrylamide gel analyses of RNA pulse-labeled after addition of the two antibiotics in synergy showed that synthesis of all major classes of RNA was progressively and uniformly inhibited.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Infect Dis. 1969 Oct;120(4):427-36 - PubMed
    1. J Bacteriol. 1969 Sep;99(3):807-14 - PubMed
    1. Annu Rev Pharmacol. 1970;10:119-42 - PubMed
    1. J Mol Biol. 1970 Sep 14;52(2):323-35 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jan;69(1):196-9 - PubMed

MeSH terms

LinkOut - more resources