Review
doi: 10.1016/S1359-6446(04)03360-4.
Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery
Affiliations
- PMID: 15808820
- DOI: 10.1016/S1359-6446(04)03360-4
Item in Clipboard
Review
Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery
Drug Discov Today.
.
Abstract
The emergence of a relatively new technique resulting from a combination of frontal affinity chromatography coupled with MS detection (FAC-MS) has extended the capabilities of MS in drug discovery and development. Its application in a broad range of biological systems, together with its label-free operation, relatively high throughput, ability to rank ligands and determine Kd, makes FAC-MS a universal tool enabling convenient and efficient screening in the identification of new potential drug leads. Here we will highlight FAC-MS screening studies and discuss where it can be applied in evaluating multiple protein-binding sites, protein-protein interactions and inactive proteins, and also in determining selectivity.
Similar articles
-
High-throughput screening for enzyme inhibitors using frontal affinity chromatography with liquid chromatography and mass spectrometry.Anal Chem. 2005 Oct 1;77(19):6125-33. doi: 10.1021/ac051131r. Anal Chem. 2005. PMID: 16194069
-
Frontal affinity chromatography in characterizing immobilized receptors.J Pharm Biomed Anal. 2011 Apr 5;54(5):911-25. doi: 10.1016/j.jpba.2010.11.040. Epub 2010 Dec 2. J Pharm Biomed Anal. 2011. PMID: 21190807 Review.
-
Frontal affinity chromatography with MS detection of EphB2 tyrosine kinase receptor. 1. Comparison with conventional ELISA.J Med Chem. 2004 Oct 7;47(21):5094-100. doi: 10.1021/jm049733a. J Med Chem. 2004. PMID: 15456253
-
Application of frontal affinity chromatography with mass spectrometry (FAC-MS) for stereospecific ligand-macromolecule interaction, detection and screening.Methods Mol Biol. 2009;572:219-30. doi: 10.1007/978-1-60761-244-5_14. Methods Mol Biol. 2009. PMID: 20694695
-
Applications of mass spectrometry in early stages of target based drug discovery.J Pharm Biomed Anal. 2006 Feb 24;40(3):528-38. doi: 10.1016/j.jpba.2005.08.038. Epub 2005 Oct 26. J Pharm Biomed Anal. 2006. PMID: 16256286 Review.
Cited by
-
Frontal affinity chromatography-mass spectrometry useful for characterization of new ligands for GPR17 receptor.J Med Chem. 2010 May 13;53(9):3489-501. doi: 10.1021/jm901691y. J Med Chem. 2010. PMID: 20394377 Free PMC article.
-
Exploring new targets and chemical space with affinity selection-mass spectrometry.Nat Rev Chem. 2021 Jan;5(1):62-71. doi: 10.1038/s41570-020-00229-2. Epub 2020 Oct 21. Nat Rev Chem. 2021. PMID: 37118102 Review.
-
Label-free affinity screening, design and synthesis of inhibitors targeting the Mycobacterium tuberculosis L-alanine dehydrogenase.PLoS One. 2022 Nov 17;17(11):e0277670. doi: 10.1371/journal.pone.0277670. eCollection 2022. PLoS One. 2022. PMID: 36395154 Free PMC article.
-
Improving success rates for lead generation using affinity binding technologies.Curr Opin Biotechnol. 2005 Dec;16(6):666-73. doi: 10.1016/j.copbio.2005.10.007. Epub 2005 Oct 28. Curr Opin Biotechnol. 2005. PMID: 16257522 Free PMC article. Review.
-
High-Throughput Native Mass Spectrometry Screening in Drug Discovery.Front Mol Biosci. 2022 Apr 14;9:837901. doi: 10.3389/fmolb.2022.837901. eCollection 2022. Front Mol Biosci. 2022. PMID: 35495635 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
