Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa
- PMID: 156923
- PMCID: PMC383662
- DOI: 10.1073/pnas.76.6.2635
Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa
Abstract
Recent studies have shown that the gene encoding the large (25 S) mitochondrial rRNA of Neurospora crassa contains an intervening sequence of 2-2.5 kilobases that is not present in the mature 25S mitochondrial rRNA. Earlier studies had provided evidence that mitochondrial rRNAs in Neurospora are synthesized via a 32S precursor RNA that contains sequences for both the mature 19S and 25S RNA species. The present work shows that the intervening sequence is not present in 32S RNA. However, we have identified two temperature-sensitive nuclear mutants that fail to excise the intervening sequence at the nonpermissive temperature (37 degrees C). When grown at 37 degrees C, the mutants show decreased ratios of 25S to 19S RNA and accumulate a novel 35S RNA that appears to consist of 25S RNA plus most or all of the intervening sequence. The mutants are allelic but can be distinguished in temperature shift-up experiments, mitochondrial rRNA processing turning off more rapidly in one than in the other. These mutants should provide powerful new tools for studying RNA processing in eukaryotic cells.
Similar articles
-
Binding of mitochondrial ribosomal proteins to a mitochondrial ribosomal precursor RNA containing a 2.3-kilobase intron.J Biol Chem. 1979 Nov 25;254(22):11746-50. J Biol Chem. 1979. PMID: 159302
-
Ribonucleic acid splicing in Neurospora Mitochondria: secondary structure of the 35S ribosomal precursor ribonucleic acid investigated by digestion with ribonuclease III and by electron microscopy.Biochemistry. 1981 May 12;20(10):2836-42. doi: 10.1021/bi00513a020. Biochemistry. 1981. PMID: 6264946
-
Split gene for mitochondrial 24S ribosomal RNA of Neurospora crassa.Cell. 1979 May;17(1):191-200. doi: 10.1016/0092-8674(79)90307-6. Cell. 1979. PMID: 156584
-
RNA splicing in Neurospora mitochondria. Characterization of new nuclear mutants with defects in splicing the mitochondrial large rRNA.Cell. 1982 Jun;29(2):517-26. doi: 10.1016/0092-8674(82)90168-4. Cell. 1982. PMID: 7116448
-
The plant mitochondrial genome and its mutants.Cell. 1983 Mar;32(3):659-61. doi: 10.1016/0092-8674(83)90051-x. Cell. 1983. PMID: 6339071 Review. No abstract available.
Cited by
-
Has the endosymbiont hypothesis been proven?Microbiol Rev. 1982 Mar;46(1):1-42. doi: 10.1128/mr.46.1.1-42.1982. Microbiol Rev. 1982. PMID: 6178009 Free PMC article. Review. No abstract available.
-
Mitochondrial DNA from Podospora anserina : IV. The large ribosomal RNA gene contains two long intervening sequences.Curr Genet. 1983 Apr;7(2):151-7. doi: 10.1007/BF00365641. Curr Genet. 1983. PMID: 24173158
-
Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.Nucleic Acids Res. 1981 Aug 11;9(15):3747-64. doi: 10.1093/nar/9.15.3747. Nucleic Acids Res. 1981. PMID: 7279671 Free PMC article.
-
Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients.J Cell Biol. 1982 Oct;95(1):267-77. doi: 10.1083/jcb.95.1.267. J Cell Biol. 1982. PMID: 6216256 Free PMC article.
-
Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa.FEBS Open Bio. 2016 Mar 14;6(4):358-68. doi: 10.1002/2211-5463.12047. eCollection 2016 Apr. FEBS Open Bio. 2016. PMID: 27239448 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
