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. 2004 Dec;74(4):517-20.
doi: 10.1002/jmv.20207.

Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody

Paul K S Chan  1 , W K To, Esther Y M Liu, T K Ng, John S Tam, Joseph J Y Sung, Jean-Michel Lacroix, Michel Houde
Affiliations

Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody

Paul K S Chan et al. J Med Virol. 2004 Dec.

Abstract

High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.

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