Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta
- PMID: 1542570
- PMCID: PMC312012
- DOI: 10.1093/nar/20.4.735
Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta
Abstract
The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol delta catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol delta is closely related to yeast pol delta and the herpes virus DNA polymerases. The levels of pol delta message were found to be induced concomitantly with DNA pol delta activity and DNA synthesis in serum restimulated proliferating IMR90 cultured cells. The human pol delta gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.
Similar articles
-
Cloning of a mouse cDNA encoding DNA polymerase delta: refinement of the homology boxes.Gene. 1993 Dec 8;134(2):191-200. doi: 10.1016/0378-1119(93)90093-i. Gene. 1993. PMID: 8262377
-
Isolation and sequence determination of the cDNA encoding DNA polymerase delta from Drosophila melanogaster.Gene. 1995 Dec 12;166(2):237-42. doi: 10.1016/0378-1119(95)00567-6. Gene. 1995. PMID: 8543168
-
Primary structure of the catalytic subunit of human DNA polymerase delta and chromosomal location of the gene.Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11197-201. doi: 10.1073/pnas.88.24.11197. Proc Natl Acad Sci U S A. 1991. PMID: 1722322 Free PMC article.
-
Cloning, chromosomal localization, and interspecies interaction of mouse DNA polymerase delta small subunit (PolD2).Genomics. 1997 Aug 15;44(1):45-51. doi: 10.1006/geno.1997.4838. Genomics. 1997. PMID: 9286699
-
DNA polymerases of parasitic protozoa.Int J Parasitol. 1994 Jul;24(4):463-76. doi: 10.1016/0020-7519(94)90136-8. Int J Parasitol. 1994. PMID: 8082975 Review. No abstract available.
Cited by
-
The 3'-->5' exonuclease associated with HeLa DNA polymerase epsilon.Chromosoma. 1992;102(1 Suppl):S142-6. doi: 10.1007/BF02451798. Chromosoma. 1992. PMID: 1337878
-
New nucleotide sequence data on the EMBL File Server.Nucleic Acids Res. 1992 May 11;20(9):2383-400. doi: 10.1093/nar/20.9.2383. Nucleic Acids Res. 1992. PMID: 1594461 Free PMC article. No abstract available.
-
Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer.Eur J Hum Genet. 2011 Mar;19(3):320-5. doi: 10.1038/ejhg.2010.216. Epub 2010 Dec 15. Eur J Hum Genet. 2011. PMID: 21157497 Free PMC article.
-
Elucidating the Inhibitory Effect of Resveratrol and Its Structural Analogs on Selected Nucleotide-Related Enzymes.Biomolecules. 2020 Aug 22;10(9):1223. doi: 10.3390/biom10091223. Biomolecules. 2020. PMID: 32842666 Free PMC article.
-
Scrutinizing Deleterious Nonsynonymous SNPs and Their Effect on Human POLD1 Gene.Genet Res (Camb). 2022 May 11;2022:1740768. doi: 10.1155/2022/1740768. eCollection 2022. Genet Res (Camb). 2022. PMID: 35620275 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
