Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody
- PMID: 15395569
- PMCID: PMC2135948
- DOI: 10.1084/jem.91.1.1
Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody
Abstract
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
Similar articles
-
Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.J Exp Med. 1955 Jul 1;102(1):49-60. doi: 10.1084/jem.102.1.49. J Exp Med. 1955. PMID: 14392240 Free PMC article.
-
Staining toxoplasma gondii with fluorescein-labelled antibody. II. A new serologic test for antibodies to Toxoplasma based upon inhibition of specific staining.J Exp Med. 1957 Jun 1;105(6):557-73. doi: 10.1084/jem.105.6.557. J Exp Med. 1957. PMID: 13428924 Free PMC article.
-
Staining Toxoplasma gondii with fluorescein-labelled antibody. I. The reaction in smears of peritoneal exudate.J Exp Med. 1957 Jun 1;105(6):549-56. doi: 10.1084/jem.105.6.549. J Exp Med. 1957. PMID: 13428923 Free PMC article.
-
Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.J Exp Med. 1961 Jul 1;114(1):89-110. doi: 10.1084/jem.114.1.89. J Exp Med. 1961. PMID: 13706641 Free PMC article.
-
Peptide immunocytochemistry.Prog Histochem Cytochem. 1981;13(4):1-85. Prog Histochem Cytochem. 1981. PMID: 6166029 Review.
Cited by
-
The immunology of experimental Chagas' disease. IV. Production of lesions in rabbits similar to those of chronic Chagas' disease in man.Am J Pathol. 1975 Jul;80(1):163-80. Am J Pathol. 1975. PMID: 808136 Free PMC article.
-
Characteization and distribution of two new foamy viruses isolated from chimpanzees.Arch Gesamte Virusforsch. 1972;38(1):38-55. doi: 10.1007/BF01241354. Arch Gesamte Virusforsch. 1972. PMID: 4626670 No abstract available.
-
[Immunfluorescence investigations in various dermatoses. IV. Proof of antigen at positive potassium bichromate pathc skin test with the direct immunofluorescence method].Arch Klin Exp Dermatol. 1968;231(3):239-49. Arch Klin Exp Dermatol. 1968. PMID: 4882699 German. No abstract available.
-
Immunological study of HB-AG carriers.Klin Wochenschr. 1975 May 15;53(10):489-90. doi: 10.1007/BF01468635. Klin Wochenschr. 1975. PMID: 1195653
-
Immunomorphology and pathogenesis of echinococcosis. III: Microscopic immunofluorescence studies in experimental alveolar echinococcosis.Infection. 1978;6(1):12-5. doi: 10.1007/BF01641084. Infection. 1978. PMID: 344225
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources