Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jun;11(6):2095-102.
doi: 10.1002/j.1460-2075.1992.tb05268.x.

Palmitoylation alters protein activity: blockade of G(o) stimulation by GAP-43

Y Sudo  1 , D Valenzuela, A G Beck-Sickinger, M C Fishman, S M Strittmatter
Affiliations

Palmitoylation alters protein activity: blockade of G(o) stimulation by GAP-43

Y Sudo et al. EMBO J. 1992 Jun.

Abstract

The addition of palmitate to cysteine residues enhances the hydrophobicity of proteins, and consequently their membrane association. Here we have investigated whether this type of fatty acylation also regulates protein-protein interactions. GAP-43 is a neuronal protein that increases guanine nucleotide exchange by heterotrimeric G proteins. Two cysteine residues near the N-terminus of GAP-43 are subject to palmitoylation, and are necessary for membrane binding as well as for G(o) activation. N-terminal peptides, which include these cysteines, stimulate G(o). Monopalmitoylation reduces, and dipalmitoylation abolishes the activity of the peptides. The activity of GAP-43 protein purified from brain also is reversibly blocked by palmitoylation. This suggests that palmitoylation controls a cycle of GAP-43 between an acylated, membrane-bound reservoir of inactive GAP-43, and a depalmitoylated, active pool of protein.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. EMBO J. 1991 Oct;10(10):2741-6 - PubMed
    1. Annu Rev Neurosci. 1989;12:127-56 - PubMed
    1. Biochim Biophys Acta. 1991 Jun 24;1078(2):147-54 - PubMed
    1. Trends Biochem Sci. 1990 Apr;15(4):139-42 - PubMed
    1. J Biol Chem. 1990 Sep 25;265(27):16644-55 - PubMed

Publication types

MeSH terms