Interaction of a common factor with ATF, Sp1, or TATAA promoter elements is required for these sequences to mediate transactivation by the adenoviral oncogene E1a
- PMID: 1531085
- PMCID: PMC364210
- DOI: 10.1128/mcb.12.2.512-517.1992
Interaction of a common factor with ATF, Sp1, or TATAA promoter elements is required for these sequences to mediate transactivation by the adenoviral oncogene E1a
Abstract
The adenovirus protein E1a stimulates transcription of both viral and cellular genes. Unlike most other transcription factors, it induces transactivation through several different promoter elements. The mechanism by which elements of diverse sequence mediate the effect of E1a is the focus of this study. Three E1a-responsive elements (an ATF site, an Sp1 site, and a TATA box containing the sequence TATAA) were studied to determine whether their interaction with a common factor is necessary for transactivation. In transfection assays, each element was used as a competitor against promoter constructs containing the other elements. The elements as competitors had no effect on basal transcription, but each competitor completely inhibited transactivation by E1a. Competitors that were not E1a responsive failed to inhibit transactivation. Therefore, either E1a itself or an E1a-inducible factor interacts with each of the elements to cause transactivation, most likely though an association with each element's specific binding protein.
Similar articles
-
Multiple transcription factor binding sites mediate adenovirus E1A transactivation.J Virol. 1989 Aug;63(8):3499-506. doi: 10.1128/JVI.63.8.3499-3506.1989. J Virol. 1989. PMID: 2545919 Free PMC article.
-
Adenovirus early region 3 promoter regulation by E1A/E1B is independent of alterations in DNA binding and gene activation of CREB/ATF and AP1.J Virol. 1990 May;64(5):2004-13. doi: 10.1128/JVI.64.5.2004-2013.1990. J Virol. 1990. PMID: 2139139 Free PMC article.
-
A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein.Cell. 1990 Jun 29;61(7):1217-24. doi: 10.1016/0092-8674(90)90686-9. Cell. 1990. PMID: 2142019
-
Regulation of adenovirus 12 E1A transcription: E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1, DP-1, cyclin A and/or RB and mediate transcriptional (auto)activation.Cell Mol Biol Res. 1993;39(8):705-16. Cell Mol Biol Res. 1993. PMID: 7951410
-
Adenovirus promoters and E1A transactivation.Annu Rev Genet. 1986;20:45-79. doi: 10.1146/annurev.ge.20.120186.000401. Annu Rev Genet. 1986. PMID: 3028247 Review. No abstract available.
Cited by
-
Nerve Growth factor regulation of cyclin D1 in PC12 cells through a p21RAS extracellular signal-regulated kinase pathway requires cooperative interactions between Sp1 and nuclear factor-kappaB.Mol Biol Cell. 2008 Jun;19(6):2566-78. doi: 10.1091/mbc.e06-12-1110. Epub 2008 Mar 26. Mol Biol Cell. 2008. PMID: 18367547 Free PMC article.
-
Vascular cell adhesion molecule 1: contrasting transcriptional control mechanisms in muscle and endothelium.Proc Natl Acad Sci U S A. 1993 May 1;90(9):3943-7. doi: 10.1073/pnas.90.9.3943. Proc Natl Acad Sci U S A. 1993. PMID: 7683412 Free PMC article.
-
The Rb family contains a conserved cyclin-dependent-kinase-regulated transcriptional repressor motif.Mol Cell Biol. 1996 Dec;16(12):7173-81. doi: 10.1128/MCB.16.12.7173. Mol Cell Biol. 1996. PMID: 8943373 Free PMC article.
-
Functional domains of the human orphan receptor ARP-1/COUP-TFII involved in active repression and transrepression.Mol Cell Biol. 1997 Sep;17(9):4914-32. doi: 10.1128/MCB.17.9.4914. Mol Cell Biol. 1997. PMID: 9271371 Free PMC article.
-
A novel application of E1A in combination therapy with EGFR-TKI treatment in breast cancer.Oncotarget. 2016 Sep 27;7(39):63924-63936. doi: 10.18632/oncotarget.11737. Oncotarget. 2016. PMID: 27590506 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
