Comparative Study
doi: 10.1292/jvms.66.529.
Use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for B virus diagnosis
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- PMID: 15187363
- DOI: 10.1292/jvms.66.529
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Comparative Study
Use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for B virus diagnosis
J Vet Med Sci.
2004 May.
Abstract
Herpesvirus papio 2 (HVP2), which infects baboons, is much more closely related genetically and antigenically to monkey B virus (BV) than to human herpes simplex virus 1(HSV1) and other related herpes viruses. The usefulness of HVP2 as an alternative test antigen in immunoblotting assays to detect BV-antibody in macaque monkey sera was assessed. Six HVP2 proteins reacted with BV-positive sera in immunoblotting. No specific bands could be detected with BV-negative sera. These results show the usefulness of HVP2 antigen as an alternative and safer antigen than authentic BV antigen in detecting BV antibody in immunoblotting.
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