GDNF promotes neuronal differentiation and dopaminergic development of mouse mesencephalic neurospheres
- PMID: 15135891
- DOI: 10.1016/j.neulet.2003.12.106
GDNF promotes neuronal differentiation and dopaminergic development of mouse mesencephalic neurospheres
Abstract
In the present study we report establishment of a neurosphere culture system derived from mouse ventral mesencephalon at embryonic day 12 and investigate effects of glial cell line-derived neurotrophic factor (GDNF) in the differentiation potential of the neurospheres. The generated neurospheres exhibit stem cell characteristics, i.e. self-renewal capacity and multipotency. Addition of exogenous GDNF resulted in neural differentiation indicated by reduced number of nestin positive cells. GDNF treatment resulted in increased numbers of beta-III-tubulin immunoreactive cells whereas glial fibrillary acidic protein immunoreactivity was not effected. Most importantly, cell numbers expressing early dopaminergic markers, Nurr1 and Ptx3, were significantly higher in GDNF-treated spheres. We conclude that GDNF promotes differentiation of mouse mesencephalic stem cells towards neuronal lineage and most notably dopaminergic development.
Similar articles
-
Effect of GDNF on differentiation of cultured ventral mesencephalic dopaminergic and non-dopaminergic calretinin-expressing neurons.Brain Res. 2005 Mar 2;1036(1-2):163-72. doi: 10.1016/j.brainres.2004.12.054. Brain Res. 2005. PMID: 15725414
-
Characterization and differentiation potential of rat ventral mesencephalic neuronal progenitor cells immortalized with SV40 large T antigen.Cell Tissue Res. 2010 Apr;340(1):29-43. doi: 10.1007/s00441-010-0933-4. Epub 2010 Feb 23. Cell Tissue Res. 2010. PMID: 20177706
-
Donor age affects differentiation of rat ventral mesencephalic stem cells.Neurosci Lett. 2005 Feb 28;375(2):101-6. doi: 10.1016/j.neulet.2004.10.083. Epub 2004 Nov 30. Neurosci Lett. 2005. PMID: 15670650
-
Generation of dopaminergic neurons from embryonic stem cells.J Neurol. 2002 Sep;249 Suppl 2:II41-4. doi: 10.1007/s00415-002-1208-0. J Neurol. 2002. PMID: 12375063 Review.
-
Development of mesencephalic dopaminergic neurons and the transforming growth factor-beta superfamily.J Neural Transm Suppl. 1995;46:209-16. J Neural Transm Suppl. 1995. PMID: 8821057 Review.
Cited by
-
Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat.Bioact Mater. 2022 Nov 23;23:300-313. doi: 10.1016/j.bioactmat.2022.11.008. eCollection 2023 May. Bioact Mater. 2022. PMID: 36439085 Free PMC article.
-
Efficient Conversion of Spermatogonial Stem Cells to Phenotypic and Functional Dopaminergic Neurons via the PI3K/Akt and P21/Smurf2/Nolz1 Pathway.Mol Neurobiol. 2015 Dec;52(3):1654-1669. doi: 10.1007/s12035-014-8966-4. Epub 2014 Nov 6. Mol Neurobiol. 2015. PMID: 25373443
-
The Non-Survival Effects of Glial Cell Line-Derived Neurotrophic Factor on Neural Cells.Front Mol Neurosci. 2017 Aug 22;10:258. doi: 10.3389/fnmol.2017.00258. eCollection 2017. Front Mol Neurosci. 2017. PMID: 28878618 Free PMC article. Review.
-
Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells.Stem Cells. 2008 Jun;26(6):1517-25. doi: 10.1634/stemcells.2008-0039. Epub 2008 Apr 3. Stem Cells. 2008. PMID: 18388303 Free PMC article.
-
Dopaminergic neurons derived from BG01V2, a variant of human embryonic stem cell line BG01.Restor Neurol Neurosci. 2008;26(6):447-58. Restor Neurol Neurosci. 2008. PMID: 19096132 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous
