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. 2004 Apr;15(4):265-76.
doi: 10.1007/s00335-003-2329-1.

Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5

Kuniya Abe  1 , Misako Yuzuriha, Michihiko Sugimoto, Minoru S H Ko, Mgavi Brathwaite, Paul Waeltz, Ramaiah Nagaraja
Affiliations

Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5

Kuniya Abe et al. Mamm Genome. 2004 Apr.

Abstract

Mice homozygous for the t(w5) allele arrest at gastrulation from defects associated with embryonic ectoderm development. The mutated gene has been genetically closely linked to the H-2K locus in the mouse MHC region, flanked by markers H-2Pb and D17Mit147. Aiming at the positional cloning of the mutated gene, we constructed a BAC contig spanning about 1 Mb of the genomic region. On the basis of our mapping and sequencing analysis of the BACs combined with public genome data, EST database searches, and gene prediction programs, we delimit the 1.06 cM of the t(w5) critical region to 750 kb, and infer 36 genes (1/20 kb) encoded in the interval. All of the 33 genes tested were confirmed as expressed in embryonic tissues by RT-PCR analyses, and in many cases by EST expression profiles as well. Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for the t(w5) embryonic lesion.

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References

    1. Genesis. 2003 Dec;37(4):162-71 - PubMed
    1. Genes Dev. 1999 Aug 1;13(15):1918-23 - PubMed
    1. Mol Cell Biol. 2000 Mar;20(5):1649-58 - PubMed
    1. Nucleic Acids Res. 1990 May 25;18(10):2887-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):773-7 - PubMed

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