The composition and structure of isolated chromosomes
- PMID: 14832432
- PMCID: PMC2147268
- DOI: 10.1085/jgp.34.5.475
The composition and structure of isolated chromosomes
Abstract
1. The preparation of isolated chromosomes from liver, kidney, and pancreas has been described. 2. It has been shown that there is no gross cytoplasmic contamination in these preparations. 3. In a microscopic study of isolated chromosomes the same chromosomes have been found in different tissues of the same organism. Since individuality is one of the main characteristics of chromosomes, there can be little doubt that the preparations do, in fact, contain isolated chromosomes. 4. A quantitative study of staining with crystal violet shows that this basic dye competes with histone for the phosphoric acid groups of the DNA in chromosomes. The displacement of histone by protamine has been demonstrated. 5. Preparation of histone-free chromosomes has been described. Removal of histone does not affect the microscopic appearance of chromosomes. 6. The non-histone or residual protein has been prepared from histone-free chromosomes. The quantity of residual protein in a preparation of chromosomes is correlated with the amount of cytoplasm in the cells from which the chromosomes were prepared. 7. The microscopic appearance of chromosomes depends upon the association of DNA with residual protein. 8. Evidence has been given that in a chromosome there are two DNA-containing nucleoproteins; in one DNA is combined with histone, and in the other it is combined with residual protein.
Similar articles
-
Protein composition of the chromosomal scaffold and interphase nuclear matrix.Chromosoma. 1985;91(2):137-44. doi: 10.1007/BF00294058. Chromosoma. 1985. PMID: 3987439
-
THE CHEMICAL COMPOSITION OF ISOLATED CHROMOSOMES.J Gen Physiol. 1947 Sep 20;31(1):7-18. doi: 10.1085/jgp.31.1.7. J Gen Physiol. 1947. PMID: 19873520 Free PMC article.
-
Some observations on protein metabolism in chromosomes of non-dividing cells.J Gen Physiol. 1955 Jan 20;38(3):415-24. doi: 10.1085/jgp.38.3.415. J Gen Physiol. 1955. PMID: 13221781 Free PMC article.
-
Evidence of differences in the desoxyribonucleoprotein complex of rapidly proliferating and non-dividing cells.J Biophys Biochem Cytol. 1955 Nov 25;1(6):531-50. doi: 10.1083/jcb.1.6.531. J Biophys Biochem Cytol. 1955. PMID: 13278365 Free PMC article.
-
ADVANCES IN THE ULTRASTRUCTURE OF THE NUCLEUS AND CHROMOSOMES.Natl Cancer Inst Monogr. 1964 May;14:33-55. Natl Cancer Inst Monogr. 1964. PMID: 14147512 Review. No abstract available.
Cited by
-
A model of the breakdown and removal of the chromatin components during Feulgen acid hydrolysis.Histochem J. 1976 Jul;8(4):419-30. doi: 10.1007/BF01003830. Histochem J. 1976. PMID: 60310
-
The surface and internal structure of metaphase chromatin.Chromosoma. 1970;29(1):74-87. doi: 10.1007/BF01183662. Chromosoma. 1970. PMID: 5461167 No abstract available.
-
A cytochemical study of the L. E. bodies of systemic lupus erythematosus. I. Nucleic acids.J Exp Med. 1957 Oct 1;106(4):575-92. doi: 10.1084/jem.106.4.575. J Exp Med. 1957. PMID: 13475615 Free PMC article.
-
The absence of histone in the bacterium Escherichia coli. I. Preparation and analysis of nucleoprotein extract.J Biophys Biochem Cytol. 1959 Jan 25;5(1):51-4. doi: 10.1083/jcb.5.1.51. J Biophys Biochem Cytol. 1959. PMID: 13630933 Free PMC article.
-
[Cytophotometric studies of DNA, histone and total protein content of epitheloid cells, tissue mast cells and of cells of plasmocytopoiesis in caseous lymph node tuberculosis].Klin Wochenschr. 1967 Nov 15;45(22):1135-42. doi: 10.1007/BF01727396. Klin Wochenschr. 1967. PMID: 4874022 German. No abstract available.
