Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Dec;20(12):1995-2000.
doi: 10.1023/b:pham.0000008048.58777.da.

Thermosensitive hydrogel as a Tgf-beta1 gene delivery vehicle enhances diabetic wound healing

Pui-Yan Lee  1 , Zhenhua Li, Leaf Huang
Affiliations

Thermosensitive hydrogel as a Tgf-beta1 gene delivery vehicle enhances diabetic wound healing

Pui-Yan Lee et al. Pharm Res. 2003 Dec.

Abstract

Purpose: To accelerate diabetic wound healing with TGF-beta1 gene delivery system using a thermosensitive hydrogel made of a triblock copolymer, PEG-PLGA-PEG.

Methods: Two 7 x 7 mm full thickness excisional wounds were created in parallel at the back of each genetically diabetic mouse. The hydrogel containing plasmid TGF-beta1 was administered to the wound and formed an adhesive film in situ. Controls were either untreated or treated with the hydrogel without DNA. We used a commercial wound dressing, Humatrix, either with or without DNA, to compare the therapeutic effect with the thermosensitive hydrogel.

Results: We found that thermosensitive hydrogel alone is slightly beneficial for reepithealization at early stage of healing (day 1-5), but significantly accelerated repithelializaion, increased cell proliferation, and organized collagen were observed in the wound bed treated with thermosensitive hydrogel containing plasmid TGF-beta1. The accelerated reepithelialization was accompanied with enhanced collagen synthesis and more organized extracellular matrix deposition. Humatrix alone or with plasmid TGF-beta1, had little effect.

Conclusions: Thermosensitive hydrogel made of PEG-PLGA-PEG triblock copolymer provides excellent wound dressing activity and delivers plasmid TGF-beta1 to promote wound healing in a diabetic mouse model.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Pediatr Surg. 2000 Feb;35(2):183-7; discussion 187-8 - PubMed
    1. Arch Pathol. 1964 Jul;78:69-72 - PubMed
    1. Gene Ther. 2002 Sep;9(18):1254-61 - PubMed
    1. Arthritis Rheum. 2000 Nov;43(11):2598-605 - PubMed
    1. Diabet Med. 2002 Jun;19(6):440-7 - PubMed

Publication types

MeSH terms