New embryo lethals in Arabidopsis thaliana: basic genetic and morphological study
- PMID: 14686610
- DOI: 10.1023/b:gene.0000003744.39599.6d
New embryo lethals in Arabidopsis thaliana: basic genetic and morphological study
Abstract
Six different mutations with defects in immature seed development have been identified during screening of a T-DNA collection of Arabidopsis thaliana. The mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development, and therefore they belong to the embryo-lethal class of mutants. The genetic and morphological studies of T-DNA mutations affecting embryo development are presented. The youngest embryos with a defect were observed at the globular stage in the VIII-64 mutation. Externally located cells, precursor of the protoderm, were characterised by abnormal cell division. VIII-41 mutation with a defect at the late globular stage was arrested at the globular-heart stage transition. VIII-111 mutation showed defect at heart stage of embryogenesis with atypical development of cotyledon primordia. The defect was associated with abnormal pattern of cell division constituting the precursor of the shoot apical meristem. In VIII-82 mutation defect in torpedo stage with asymmetric cotyledons was observed. Cotyledon stage of embryos and chlorophyll defect were observed in VIII-75 mutant. Abnormal suspensor consisting of two columns of cells was observed in 280-4-4 mutation. Newly identified embryo-lethals can serve as starting material for more detailed genetic and molecular studies.
Similar articles
-
Mutations of Arabidopsis thaliana that transform leaves into cotyledons.Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10209-14. doi: 10.1073/pnas.94.19.10209. Proc Natl Acad Sci U S A. 1997. PMID: 9294189 Free PMC article.
-
An Arabidopsis embryonic lethal mutant with reduced expression of alanyl-tRNA synthetase gene.Cell Res. 1998 Jun;8(2):119-34. doi: 10.1038/cr.1998.12. Cell Res. 1998. PMID: 9669027
-
Arabidopsis emb175 and other ppr knockout mutants reveal essential roles for pentatricopeptide repeat (PPR) proteins in plant embryogenesis.Planta. 2005 Jun;221(3):424-36. doi: 10.1007/s00425-004-1452-x. Epub 2005 Jan 13. Planta. 2005. PMID: 15647901
-
Pattern formation in the Arabidopsis embryo: a genetic perspective.Philos Trans R Soc Lond B Biol Sci. 1995 Oct 30;350(1331):19-25. doi: 10.1098/rstb.1995.0132. Philos Trans R Soc Lond B Biol Sci. 1995. PMID: 8577846 Review.
-
Future of early embryogenesis studies in Arabidopsis thaliana.C R Acad Sci III. 2001 Jun;324(6):569-73. doi: 10.1016/s0764-4469(01)01327-0. C R Acad Sci III. 2001. PMID: 11455880 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources