A new austenitic stainless steel with negligible nickel content: an in vitro and in vivo comparative investigation
- PMID: 14559006
- DOI: 10.1016/s0142-9612(03)00416-2
A new austenitic stainless steel with negligible nickel content: an in vitro and in vivo comparative investigation
Abstract
New nickel (Ni)-reduced stainless-steel metals have recently been developed to avoid sensitivity to Ni. In the present study, an austenitic Ni-reduced SSt named P558 (P558, Böhler, Milan, Italy) was studied in vitro on primary osteoblasts and in vivo after bone implantation in the sheep tibia, and was compared to ISO 5832-9 SSt (SSt) and Ti6Al4V. Cells were cultured directly on P558 and Ti6Al4V. Cells cultured on polystyrene were used as controls. Osteoblast proliferation, viability and synthetic activity were evaluated at 72 h by assaying WST1, alkaline phosphatase activity (ALP), nitric oxide, pro-collagen I (PICP), osteocalcin (OC), transforming growth factor-beta1 (TGFbeta-1) and interleukin-6 (IL-6) after 1.25(OH)2D3 stimulation. Under general anaesthesia, four sheep were submitted for bilateral tibial implantation of P558, SSt and Ti6Al4V rods. In vitro results demonstrated that the effect of P558 on osteoblast viability, PICP, TGF beta-1, tumor necrosis factor-alpha production did not significantly differ from that exerted by Ti6Al4V and controls. Furthermore, P558 enhanced osteoblast differentiation, as confirmed by ALP and OC levels, and reduced IL-6 production. At 26 weeks, the bone-to-implant contact was higher in P558 than in SSt (28%, p<0.005) and Ti6Al4V (4%, p<0.05), and was higher in Ti6Al4V than in SSt (22%, p<0.005). The tested materials did not affect bone microhardness in pre-existing host bone as evidenced by the measurements taken at 1000 microm from the bone-biomaterial interface (F=1.89, ns). At the bone-biomaterial interface the lowest HV value was found for SSt, whereas no differences in HV were observed between materials (F=1.55, ns). The current findings demonstrate P558 biocompatibility both in vitro and in vivo, and osteointegration processes are shown to be significantly improved by P558 as compared to the other materials tested.
Similar articles
-
A new austenitic stainless steel with a negligible amount of nickel: an in vitro study in view of its clinical application in osteoporotic bone.J Biomed Mater Res B Appl Biomater. 2004 Oct 15;71(1):30-7. doi: 10.1002/jbm.b.30068. J Biomed Mater Res B Appl Biomater. 2004. PMID: 15368225
-
Biomaterials in orthopedic surgery: effects of a nickel-reduced stainless steel on in vitro proliferation and activation of human osteoblasts.Int J Artif Organs. 2003 Oct;26(10):952-7. doi: 10.1177/039139880302601013. Int J Artif Organs. 2003. PMID: 14636013
-
Soft tissue response to a new austenitic stainless steel with a negligible nickel content.Int J Artif Organs. 2005 Oct;28(10):1003-11. doi: 10.1177/039139880502801007. Int J Artif Organs. 2005. PMID: 16288438
-
P2000 - A high-nitrogen austenitic steel for application in bone surgery.PLoS One. 2019 Mar 26;14(3):e0214384. doi: 10.1371/journal.pone.0214384. eCollection 2019. PLoS One. 2019. PMID: 30913254 Free PMC article.
-
A review on nickel-free nitrogen containing austenitic stainless steels for biomedical applications.Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3563-75. doi: 10.1016/j.msec.2013.06.002. Epub 2013 Jun 12. Mater Sci Eng C Mater Biol Appl. 2013. PMID: 23910251 Review.
Cited by
-
Multifaceted Materials for Enhanced Osteogenesis and Antimicrobial Properties on Bioplastic Polyetheretherketone Surfaces: A Review.ACS Omega. 2024 Apr 12;9(16):17784-17807. doi: 10.1021/acsomega.4c00923. eCollection 2024 Apr 23. ACS Omega. 2024. PMID: 38680314 Free PMC article. Review.
-
Performance of Austenitic High-Nitrogen Steels under Gross Slip Fretting Corrosion in Bovine Serum.J Funct Biomater. 2024 Apr 18;15(4):110. doi: 10.3390/jfb15040110. J Funct Biomater. 2024. PMID: 38667567 Free PMC article.
-
Bioactivity, Cytotoxicity, and Tribological Studies of Nickel-Free Austenitic Stainless Steel Obtained via Powder Metallurgy Route.Materials (Basel). 2023 Dec 14;16(24):7637. doi: 10.3390/ma16247637. Materials (Basel). 2023. PMID: 38138779 Free PMC article.
-
Mechanical Behavior of Austenitic Steel under Multi-Axial Cyclic Loading.Materials (Basel). 2023 Feb 6;16(4):1367. doi: 10.3390/ma16041367. Materials (Basel). 2023. PMID: 36836997 Free PMC article.
-
Biological Safety Evaluation and Surface Modification of Biocompatible Ti-15Zr-4Nb Alloy.Materials (Basel). 2021 Feb 4;14(4):731. doi: 10.3390/ma14040731. Materials (Basel). 2021. PMID: 33557312 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous