Caspase-independent cell engulfment mirrors cell death pattern in Drosophila embryos
- PMID: 14534140
- DOI: 10.1242/dev.00824
Caspase-independent cell engulfment mirrors cell death pattern in Drosophila embryos
Abstract
Programmed cell death plays an essential role during Drosophila embryonic development. A stereotypic series of cellular changes occur during apoptosis, most of which are initiated by a caspase cascade that is triggered by a trio of proteins, RPR, HID and GRIM. The final step in apoptosis is engulfment of the cell corpse. To monitor cell engulfment in vivo, we developed a fluorogenic beta-galactosidase substrate that is cleaved by an endogenous, lysosomal beta-galactosidase activity. The pattern of cell engulfment in wild-type embryos correlated well with the known pattern of apoptosis. Surprisingly, the pattern of cell engulfment persisted in apoptosis-deficient embryos. We provide evidence for a caspase-independent engulfment process that affects the majority of cells expected to die in developing Drosophila embryos.
Similar articles
-
Detection of apoptosis in Drosophila.Methods Mol Biol. 2004;282:191-205. doi: 10.1385/1-59259-812-9:191. Methods Mol Biol. 2004. PMID: 15105566
-
Methods and protocols for studying cell death in Drosophila.Methods Enzymol. 2008;446:17-37. doi: 10.1016/S0076-6879(08)01602-9. Methods Enzymol. 2008. PMID: 18603114
-
Programmed cell death in the embryonic central nervous system of Drosophila melanogaster.Development. 2007 Jan;134(1):105-16. doi: 10.1242/dev.02707. Development. 2007. PMID: 17164416
-
The genetics of cell death: approaches, insights and opportunities in Drosophila.Nat Rev Genet. 2004 Dec;5(12):911-22. doi: 10.1038/nrg1491. Nat Rev Genet. 2004. PMID: 15573123 Review.
-
Death without caspases, caspases without death.Trends Cell Biol. 2004 Apr;14(4):184-93. doi: 10.1016/j.tcb.2004.03.002. Trends Cell Biol. 2004. PMID: 15066636 Review.
Cited by
-
Characterization of the activity of β-galactosidase from Escherichia coli and Drosophila melanogaster in fixed and non-fixed Drosophila tissues.Biochim Open. 2016 Jun 22;3:1-7. doi: 10.1016/j.biopen.2016.06.001. eCollection 2016 Dec. Biochim Open. 2016. PMID: 29450125 Free PMC article.
-
Six-microns-under acts upstream of Draper in the glial phagocytosis of apoptotic neurons.Cell. 2008 May 2;133(3):498-509. doi: 10.1016/j.cell.2008.02.052. Cell. 2008. PMID: 18455990 Free PMC article.
-
Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.PLoS Biol. 2012;10(3):e1001288. doi: 10.1371/journal.pbio.1001288. Epub 2012 Mar 20. PLoS Biol. 2012. PMID: 22448145 Free PMC article.
-
The matrix protein Tiggrin regulates plasmatocyte maturation in Drosophila larva.Development. 2017 Jul 1;144(13):2415-2427. doi: 10.1242/dev.149641. Epub 2017 May 19. Development. 2017. PMID: 28526755 Free PMC article.
-
pHMA, a pH-sensitive GFP reporter for cell engulfment, in Drosophila embryos, tissues, and cells.Dev Dyn. 2010 Feb;239(2):559-73. doi: 10.1002/dvdy.22180. Dev Dyn. 2010. PMID: 20014104 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
