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. 1992 Sep-Oct;28A(9-10):615-20.
doi: 10.1007/BF02631036.

Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture

J M Fraslin  1 , L Touquette, M Douaire, Y Menezo, J C Guillemot, J Mallard
Affiliations

Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture

J M Fraslin et al. In Vitro Cell Dev Biol. 1992 Sep-Oct.

Abstract

Adult chicken hepatocytes were obtained by an adaptation of the two step in situ collagenase perfusion. Usually 0.5 to 1 x 10(9) cells were obtained, with 75 to 95% viability. Hepatocytes attached within 2 h when plated on plastic cell culture dishes and spread in 4 h, surviving for several months in a specific serum-free medium. These cells retained a typical parenchymal cell morphology and the ability to produce a specific protein (albumin) throughout the culture period. We hereby provide a suitable model for studying hepatic metabolism in birds.

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References

    1. J Biol Chem. 1984 Aug 25;259(16):10228-31 - PubMed
    1. Mol Cell Biol. 1988 Aug;8(8):3338-44 - PubMed
    1. Nature. 1982 Sep 9;299(5879):165-7 - PubMed
    1. Exp Cell Res. 1973 Dec;82(2):391-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 May;82(10):3252-6 - PubMed

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