Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov;11(11):3911-9.
doi: 10.1002/j.1460-2075.1992.tb05484.x.

Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma

L Rönnstrand  1 , S Mori, A K Arridsson, A Eriksson, C Wernstedt, U Hellman, L Claesson-Welsh, C H Heldin
Affiliations

Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma

L Rönnstrand et al. EMBO J. 1992 Nov.

Abstract

Two novel sites of autophosphorylation were localized to the C-terminal tail of the PDGF beta-receptor. To evaluate the importance of these phosphorylation sites, receptor mutants in which Tyr1009, Tyr1021 or both were replaced with phenylalanine residues, were expressed in porcine aortic endothelial (PAE) cells. These mutants were similar to the wild type receptor with regard to protein tyrosine kinase activity and ability to induce mitogenicity in response to PDGF-BB. However, both the Y1009F and Y1021F mutants showed a decreased ability to mediate association with and the tyrosine phosphorylation of phospholipase C-gamma (PLC-gamma) compared to the wild type PDGF beta-receptor; in the case of the Y1009F/Y1021F double mutant, no association or phosphorylation of PLC-gamma could be detected. These data show that tyrosine phosphorylation of PLC-gamma is dependent on autophosphorylation of the PDGF beta-receptor at Tyr1009 and Tyr1021.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 1990 Mar 5;265(7):3944-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2023-7 - PubMed
    1. J Biol Chem. 1991 Nov 5;266(31):21158-64 - PubMed
    1. Cell. 1990 Aug 10;62(3):481-92 - PubMed
    1. J Cell Biol. 1975 Dec;67(3):835-51 - PubMed

MeSH terms