L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli
- PMID: 13890280
- PMCID: PMC277779
- DOI: 10.1128/jb.84.1.137-146.1962
L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli
Abstract
Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ara-53 and ara-139, are deficient in the enzyme l-ribulose 5-phosphate 4-epimerase; ara-53, further analyzed, accumulates large quantities of l-ribulose 5-phosphate when incubated with l-arabinose. The mutant sites are closely linked to the left of the previously ordered l-arabinose mutant sites, and probably represent the structural gene for l-ribulose 5-phosphate 4-epimerase (gene D) in the l-arabinose operon. The inducible levels of l-arabinose isomerase and l-ribulose 5-phosphate 4-epimerase vary correspondingly as a result of mutation in the structural gene for l-ribulokinase (gene B), further substantiating the dual structural and regulatory function of this gene locus. Ara-53 and ara-139 are strongly inhibited by l-arabinose and give rise to l-arabinose-resistant mutants. The one resistant mutant analyzed still lacks the 4-epimerase but is deficient in l-ribulokinase and has increased l-arabinose isomerase activity, a characteristic of a type of mutation in the B gene. It is proposed that accumulation of l-ribulose 5-phosphate is responsible for the inhibition, and that mutation to resistance will involve mutation in the A, B, C, permease, or repressor genes, thus providing a direct method for isolating these types of l-arabinose-negative mutants. Glucose prevents and cures the l-arabinose inhibition.
Similar articles
-
Ribose-5-phosphate isomerase and ribulose-5-phosphate kinase show apparent specificity for a specific ribulose 5-phosphate species.FEBS Lett. 1987 Feb 9;212(1):45-8. doi: 10.1016/0014-5793(87)81553-3. FEBS Lett. 1987. PMID: 3026853
-
Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli.Appl Microbiol Biotechnol. 2007 Oct;76(5):1051-7. doi: 10.1007/s00253-007-1084-8. Epub 2007 Jul 10. Appl Microbiol Biotechnol. 2007. PMID: 17619876
-
Positive control of enzyme synthesis by gene C in the L-arabinose system.J Bacteriol. 1965 Oct;90(4):946-57. doi: 10.1128/jb.90.4.946-957.1965. J Bacteriol. 1965. PMID: 5321403 Free PMC article.
-
A review on l-ribose isomerases for the biocatalytic production of l-ribose and l-ribulose.Food Res Int. 2021 Jul;145:110409. doi: 10.1016/j.foodres.2021.110409. Epub 2021 May 11. Food Res Int. 2021. PMID: 34112412 Review.
-
Metabolism of L-arabinose in plants.J Plant Res. 2016 Sep;129(5):781-792. doi: 10.1007/s10265-016-0834-z. Epub 2016 May 24. J Plant Res. 2016. PMID: 27220955 Free PMC article. Review.
Cited by
-
Acquisition of ability to utilize Xylitol: disadvantages of a constitutive catabolic pathway in Escherichia coli.J Bacteriol. 1978 May;134(2):501-5. doi: 10.1128/jb.134.2.501-505.1978. J Bacteriol. 1978. PMID: 207668 Free PMC article.
-
Utilization of gluconate by Escherichia coli. Uptake of D-gluconate by a mutant impaired in gluconate kinase activity and by membrane vesicles derived therefrom.Biochem J. 1974 May;140(2):193-203. doi: 10.1042/bj1400193. Biochem J. 1974. PMID: 4375960 Free PMC article.
-
COORDINATE VARIATIONS IN INDUCED SYNTHESES OF ENZYMES ASSOCIATED WITH MUTATIONS IN A STRUCTURAL GENE.Proc Natl Acad Sci U S A. 1963 Oct;50(4):696-702. doi: 10.1073/pnas.50.4.696. Proc Natl Acad Sci U S A. 1963. PMID: 14077500 Free PMC article. No abstract available.
-
Properties of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase.J Bacteriol. 1966 Aug;92(2):470-6. doi: 10.1128/jb.92.2.470-476.1966. J Bacteriol. 1966. PMID: 16562137 Free PMC article.
-
Comparison of a forward and a reverse mutation assay in Salmonella typhimurium measuring L-arabinose resistance and histidine prototrophy.EMBO J. 1984 Jun;3(6):1435-40. doi: 10.1002/j.1460-2075.1984.tb01989.x. EMBO J. 1984. PMID: 6378623 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
