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Comparative Study
. 1992 Jul;32(7):811-7.
doi: 10.1002/bip.360320709.

Spectroscopic study of the interaction between poly-(9-vinyladenine) and single or multistrand RNA

E Yashima  1 , T Tajima, N Miyauchi, M Akashi
Affiliations
Comparative Study

Spectroscopic study of the interaction between poly-(9-vinyladenine) and single or multistrand RNA

E Yashima et al. Biopolymers. 1992 Jul.

Abstract

The interaction between poly(9-vinyladenine) (PVAd) and poly[r(U)] was investigated by means of uv, CD, 1H-, and 31P-nmr spectroscopies. The interaction was dependent on the molecular weight of PVAd determined by uv and CD spectroscopies. Based on imino proton nmr, it was clearly found that PVAd formed the complex with poly[r(U)] by complementary hydrogen bonding. The interaction of PVAd with double- and triple-stranded helices of RNA was also investigated by uv melting behavior and 31P-nmr spectroscopy. The results suggested that PVAd could not interact with the double-stranded poly[r(A)].poly[r(U)] but did with the triple-stranded RNA.

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