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Comparative Study
. 1992 Jun;11(6):2151-7.
doi: 10.1002/j.1460-2075.1992.tb05274.x.

Cloning by functional complementation of a mouse cDNA encoding a homologue of CDC25, a Saccharomyces cerevisiae RAS activator

E Martegani  1 , M Vanoni, R Zippel, P Coccetti, R Brambilla, C Ferrari, E Sturani, L Alberghina
Affiliations
Comparative Study

Cloning by functional complementation of a mouse cDNA encoding a homologue of CDC25, a Saccharomyces cerevisiae RAS activator

E Martegani et al. EMBO J. 1992 Jun.

Abstract

In the yeast Saccharomyces cerevisiae genetic and biochemical evidence indicates that the product of the CDC25 gene activates the RAS/adenylyl cyclase/protein kinase A pathway by acting as a guanine nucleotide protein. Here we report the isolation of a mouse brain cDNA homologous to CDC25. The mouse cDNA, called CDC25Mm, complements specifically point mutations and deletion/disruptions of the CDC25 gene. In addition, it restores the cAMP levels and CDC25-dependent glucose-induced cAMP signalling in a yeast strain bearing a disruption of the CDC25 gene. The CDC25Mm-encoded protein is 34% identical with the catalytic carboxy terminal part of the CDC25 protein and shares significant homology with other proteins belonging to the same family. The protein encoded by CDC25Mm, prepared as a glutathione S-transferase fusion in Escherichia coli cells, activates adenylyl cyclase in yeast membranes in a RAS2-dependent manner. Northern blot analysis of mouse brain poly(A)+ RNA reveals two major transcripts of approximately 1700 and 5200 nucleotides. Transcripts were found also in mouse heart and at a lower level in liver and spleen.

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References

    1. Science. 1985 Apr 12;228(4696):179-84 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Oct;87(20):8008-12 - PubMed
    1. Cell. 1991 Jun 28;65(7):1225-31 - PubMed
    1. Cell. 1986 Jun 20;45(6):781-2 - PubMed
    1. Mol Cell Biol. 1990 Sep;10(9):4518-23 - PubMed

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