Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Feb;11(2):449-56.
doi: 10.1002/j.1460-2075.1992.tb05074.x.

Import of cytochrome c heme lyase into mitochondria: a novel pathway into the intermembrane space

R Lill  1 , R A Stuart, M E Drygas, F E Nargang, W Neupert
Affiliations

Import of cytochrome c heme lyase into mitochondria: a novel pathway into the intermembrane space

R Lill et al. EMBO J. 1992 Feb.

Abstract

Cytochrome c heme lyase (CCHL) catalyses the covalent attachment of the heme group to apocytochrome c during its import into mitochondria. The enzyme is membrane-associated and is located within the intermembrane space. The precursor of CCHL synthesized in vitro was efficiently translocated into isolated mitochondria from Neurospora crassa. The imported CCHL, like the native protein, was correctly localized to the intermembrane space, where it was membrane-bound. As with the majority of mitochondrial precursor proteins, CCHL uses the MOM19-GIP receptor complex in the outer membrane for import. In contrast to proteins taking the general import route, CCHL was imported independently of both ATP-hydrolysis and an electrochemical potential as external energy sources. CCHL which lacks a cleavable signal sequence apparently does not traverse the inner membrane to reach the intermembrane space; rather, it translocates through the outer membrane only. Thus, CCHL represents an example of a novel, 'non-conservative' import pathway into the intermembrane space, thereby also showing that the import apparatus in the outer membrane acts separately from the import machinery in the inner membrane.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1985 Nov;43(1):339-50 - PubMed
    1. Cell. 1987 Dec 24;51(6):1027-37 - PubMed
    1. EMBO J. 1987 Sep;6(9):2635-42 - PubMed
    1. EMBO J. 1985 Nov;4(11):2819-25 - PubMed
    1. EMBO J. 1986 Jun;5(6):1335-42 - PubMed

Publication types

MeSH terms