The specificity of recognition of a cytotoxic T lymphocyte epitope
- PMID: 1370414
- DOI: 10.1002/eji.1830220128
The specificity of recognition of a cytotoxic T lymphocyte epitope
Abstract
An Epstein-Barr virus (EBV)-specific CD8+ cytotoxic T lymphocyte (CTL) clone (LC13) was shown to recognize the minimal peptide determinant FLRGRAYGL from the EBNA 3 antigen of the BL74 strain of EBV. The equivalent epitope from the B95-8 strain (FLRGRAYGI) is not recognized when endogenously presented and the peptide is 15-fold less active than FLRGRAYGL. A replacement set of peptides was synthesized in which each residue within FLRGRAYGL was sequentially replaced with all other genetically coded amino acids. These peptides were tested for their ability to sensitize target cells to lysis by LC13. Of the 171 single-amino acid replacement peptides only 15 were more active than the peptide FLRGRAYGI. Five peptides had significantly greater activity than FLRGRAYGL and a peptide incorporating the most active of these single-amino acid substitutions (HIRGRAYSL) induced lysis at concentrations approximately 30-fold less than FLRGRAYGL. Simplified theoretical calculations based on this study suggest that CTL LC13 has a specificity for its target epitope of 1 in 4.7 x 10(10). This represents the first complete analysis of the role of single amino acids within a minimum epitope on the specificity of CTL recognition.
Similar articles
-
Modification of the carboxyl terminal group affects replacement set analysis of a cytotoxic T cell epitope.Pept Res. 1995 Sep-Oct;8(5):258-62. Pept Res. 1995. PMID: 8589547
-
Effect of point mutations in the native simian virus 40 tumor antigen, and in synthetic peptides corresponding to the H-2Db-restricted epitopes, on antigen presentation and recognition by cytotoxic T lymphocyte clones.J Immunol. 1992 May 15;148(10):3012-20. J Immunol. 1992. PMID: 1374447
-
Cross-reactive memory T cells for Epstein-Barr virus augment the alloresponse to common human leukocyte antigens: degenerate recognition of major histocompatibility complex-bound peptide by T cells and its role in alloreactivity.Eur J Immunol. 1997 Jul;27(7):1726-36. doi: 10.1002/eji.1830270720. Eur J Immunol. 1997. PMID: 9247584
-
Cross-recognition of a mouse H-2-peptide complex by human HLA-restricted cytotoxic T cells.Eur J Immunol. 1990 Mar;20(3):659-64. doi: 10.1002/eji.1830200329. Eur J Immunol. 1990. PMID: 1690661
-
Sequence variation of cytotoxic T cell epitopes in different isolates of Epstein-Barr virus.Eur J Immunol. 1992 Jan;22(1):183-9. doi: 10.1002/eji.1830220127. Eur J Immunol. 1992. PMID: 1370413
Cited by
-
Advances in T-cell epitope engineering.Front Immunol. 2013 Jun 5;4:133. doi: 10.3389/fimmu.2013.00133. eCollection 2013. Front Immunol. 2013. PMID: 23761792 Free PMC article. No abstract available.
-
T cell receptor repertoire for a viral epitope in humans is diversified by tolerance to a background major histocompatibility complex antigen.J Exp Med. 1995 Dec 1;182(6):1703-15. doi: 10.1084/jem.182.6.1703. J Exp Med. 1995. PMID: 7500015 Free PMC article.
-
Selective interference with class I major histocompatibility complex presentation of the major immediate-early protein following infection with human cytomegalovirus.J Virol. 1993 Jun;67(6):3461-9. doi: 10.1128/JVI.67.6.3461-3469.1993. J Virol. 1993. PMID: 8388509 Free PMC article.
-
RelB nuclear translocation regulates B cell MHC molecule, CD40 expression, and antigen-presenting cell function.Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11421-6. doi: 10.1073/pnas.97.21.11421. Proc Natl Acad Sci U S A. 2000. PMID: 11027342 Free PMC article.
-
Phase I trial of a CD8+ T-cell peptide epitope-based vaccine for infectious mononucleosis.J Virol. 2008 Feb;82(3):1448-57. doi: 10.1128/JVI.01409-07. Epub 2007 Nov 21. J Virol. 2008. PMID: 18032491 Free PMC article. Clinical Trial.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
