Variable composition of cytochrome b6-f particles
- PMID: 136131
- DOI: 10.1515/znc-1976-9-1021
Variable composition of cytochrome b6-f particles
Abstract
Cytochrome b6-f particles were prepared from chloroplasts of spinach and the heterokont alga Bumilleriopsis filiformis using digitonin in comparison with non-ionic detergents. The cytochrome b6-f particles are solubilized in different amounts according to chemical structure and HLB value of the detergent (see Griffin, J. Soc. Cosmet. Chem. 1, 311 [1949]). Also the ratio of the two cytochromes in the isolated particle depends on the detergent used. In case of spinach, it varies from Cyt b6:f=1.5:1 to 4.9:1. These investigations do not strengthen the idea that a cytochrome b6-f complex with a stoichiometric composition is present in the photosynthetic membrane. Application of different detergents is a means for selective solubilization of thylakoid membrane components.
Similar articles
-
Differential detergent-solubilization of integral thylakoid membrane complexes in spinach chloroplasts. Localization of photosystem II, cytochrome b6-f complex and photosystem I.Eur J Biochem. 1986 Oct 15;160(2):389-93. doi: 10.1111/j.1432-1033.1986.tb09983.x. Eur J Biochem. 1986. PMID: 3533538
-
Large-scale purification of active cytochrome b6/f complex from spinach chloroplasts.Arch Biochem Biophys. 1987 Feb 1;252(2):655-61. doi: 10.1016/0003-9861(87)90071-3. Arch Biochem Biophys. 1987. PMID: 3813555
-
Identification of the polypeptides in the cytochrome b6/f complex from spinach chloroplasts with redox-center-carrying subunits.J Bioenerg Biomembr. 1982 Dec;14(5-6):405-24. doi: 10.1007/BF00743067. J Bioenerg Biomembr. 1982. PMID: 6819297
-
Reconstitution of cytochrome f/b6 and CF0-CF1 ATP synthetase complexes into phospholipid and galactolipid liposomes.J Cell Biol. 1983 Aug;97(2):301-10. doi: 10.1083/jcb.97.2.301. J Cell Biol. 1983. PMID: 6193124 Free PMC article.
-
Implications of cytochrome b6/f location for thylakoidal electron transport.J Bioenerg Biomembr. 1986 Oct;18(5):419-36. doi: 10.1007/BF00743013. J Bioenerg Biomembr. 1986. PMID: 3533910 Review.