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. 1992 Nov 25;20(22):6015-21.
doi: 10.1093/nar/20.22.6015.

The E2 binding sites determine the efficiency of replication for the origin of human papillomavirus type 18

M Remm  1 , R Brain, J R Jenkins
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Free PMC article

The E2 binding sites determine the efficiency of replication for the origin of human papillomavirus type 18

M Remm et al. Nucleic Acids Res. .
Free PMC article

Abstract

Human papillomaviruses (HPV-s) have been shown to possess transforming and immortalizing activity for many different, mainly keratinocyte cell lines and they have been detected in 90% of anogenital cancer tissues, which suggests a causative role in the induction of anogenital and other tumours. We have exploited a quantitative assay to identify and characterize the origin of replication of the human papillomavirus type 18 (HPV-18), one of the most prevalent types in the high-risk HPV group. Replication of HPV origin fragments was studied transiently by cotransfection with a protein expression vector providing replication proteins E1 and E2. We have localized the HPV-18 origin to nucleotides 7767-119. This region contains three E2 binding sites and an essential A/T rich DNA region (nucleotides 9-35) that is partly homologous to the E1 binding site found in bovine papillomavirus type 1 (BPV-1) genome. At least one of the three E2 binding sites was absolutely required for origin function; addition of other E2 sites had cooperative stimulating effect. This is the first quantitative analysis of the E2 binding sites for papillomavirus replication.

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References

    1. J Virol. 1986 Nov;60(2):626-34 - PubMed
    1. Trends Genet. 1990 Sep;6(9):300-4 - PubMed
    1. Mol Cell Biol. 1986 Oct;6(10):3513-22 - PubMed
    1. J Virol. 1987 Jul;61(7):2128-37 - PubMed
    1. J Mol Biol. 1987 Feb 20;193(4):599-608 - PubMed

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