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. 1992 Jul 31;186(2):963-9.
doi: 10.1016/0006-291x(92)90840-h.

DNA supercoiling facilitates the assembly of transcriptionally active chromatin on the adenovirus major late promoter

R Ohba  1 , H Tabuchi, S Hirose
Affiliations

DNA supercoiling facilitates the assembly of transcriptionally active chromatin on the adenovirus major late promoter

R Ohba et al. Biochem Biophys Res Commun. .

Abstract

Assembly of nucleosomes on the adenovirus major late promoter blocked initiation of transcription by RNA polymerase II. However, the formation of transcription preinitiation complexes prevented subsequent assembly of promoter sequences into nucleosomes and allowed transcription on the chromatin templates. When the formation of preinitiation complexes was in competition with nucleosome assembly, transcription on linear or relaxed closed circular DNA was inactivated by nucleosome assembly over the promoter region. However, transcription on partially supercoiled DNA (mean superhelical density of -0.036) remained active because the rapid formation of preinitiation complexes prevented subsequent assembly of promoter sequences into nucleosomes.

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