Packaging system for rapid production of murine leukemia virus vectors with variable tropism
- PMID: 1321291
- PMCID: PMC241381
- DOI: 10.1128/JVI.66.8.5110-5113.1992
Packaging system for rapid production of murine leukemia virus vectors with variable tropism
Abstract
A method for rapidly producing helper-free murine leukemia virus (MLV) without using packaging cell lines is described. Viruses bearing ecotropic or amphotropic MLV or Rous sarcoma virus envelope glycoprotein and containing various retroviral vector genomes have been prepared with titers 30 to 40-fold higher than those produced by transient transfection of standard packaging cells. This system can be used to alter the cellular tropism of MLV by incorporating other envelope glycoproteins and to prepare retroviral vector stocks without establishing stable producer cell lines. This method will be particularly useful for preparing viruses that encode toxic proteins and for the rapid analysis of panels of mutant envelope glycoproteins.
Similar articles
-
Adaptation of chimeric retroviruses in vitro and in vivo: isolation of avian retroviral vectors with extended host range.J Virol. 2001 Jun;75(11):4973-83. doi: 10.1128/JVI.75.11.4973-4983.2001. J Virol. 2001. PMID: 11333876 Free PMC article.
-
Gene transfer into mammalian cells by a Rous sarcoma virus-based retroviral vector with the host range of the amphotropic murine leukemia virus.J Virol. 1996 Jun;70(6):3922-9. doi: 10.1128/JVI.70.6.3922-3929.1996. J Virol. 1996. PMID: 8648729 Free PMC article.
-
Construction of replication-competent oncolytic retroviral vectors expressing R peptide-truncated 10A1 envelope glycoprotein.J Virol Methods. 2019 Jun;268:32-36. doi: 10.1016/j.jviromet.2019.03.008. Epub 2019 Mar 18. J Virol Methods. 2019. PMID: 30898575
-
Preparation of pseudotyped retroviral vector.Methods Mol Med. 2002;69:149-59. doi: 10.1385/1-59259-141-8:149. Methods Mol Med. 2002. PMID: 11987774 Review. No abstract available.
-
Gammaretroviral vectors: biology, technology and application.Viruses. 2011 Jun;3(6):677-713. doi: 10.3390/v3060677. Epub 2011 Jun 3. Viruses. 2011. PMID: 21994751 Free PMC article. Review.
Cited by
-
In vivo analysis of human T-cell leukemia virus type 1 reverse transcription accuracy.J Virol. 2000 Oct;74(20):9525-31. doi: 10.1128/jvi.74.20.9525-9531.2000. J Virol. 2000. PMID: 11000222 Free PMC article.
-
E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.Int J Biol Sci. 2010 Jul 4;6(4):382-95. doi: 10.7150/ijbs.6.382. Int J Biol Sci. 2010. PMID: 20616879 Free PMC article.
-
CCAAT displacement protein regulates nuclear factor-kappa beta-mediated chemokine transcription in melanoma cells.Melanoma Res. 2007 Apr;17(2):91-103. doi: 10.1097/CMR.0b013e3280a60888. Melanoma Res. 2007. PMID: 17496784 Free PMC article.
-
Cysteines flanking the internal fusion peptide are required for the avian sarcoma/leukosis virus glycoprotein to mediate the lipid mixing stage of fusion with high efficiency.J Virol. 2008 Mar;82(6):3131-4. doi: 10.1128/JVI.02266-07. Epub 2008 Jan 9. J Virol. 2008. PMID: 18184714 Free PMC article.
-
Recruitment of SH2-containing protein tyrosine phosphatase SHP-1 to the interleukin 2 receptor; loss of SHP-1 expression in human T-lymphotropic virus type I-transformed T cells.Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3845-50. doi: 10.1073/pnas.95.7.3845. Proc Natl Acad Sci U S A. 1998. PMID: 9520455 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
