Platelet-derived growth factor stimulates synthesis of PtdIns(3,4,5)P3 by activating a PtdIns(4,5)P2 3-OH kinase
- PMID: 1319558
- DOI: 10.1038/358157a0
Platelet-derived growth factor stimulates synthesis of PtdIns(3,4,5)P3 by activating a PtdIns(4,5)P2 3-OH kinase
Abstract
Although the hormone-stimulated synthesis of 3-phosphorylated inositol lipids is known to form an intracellular signalling system, there is no consensus on the crucial receptor-regulated event in this pathway and it is still not clear which of the intermediates represent potential output signals. We show here that the key step in the synthesis of 3-phosphorylated inositol lipids in 3T3 cells stimulated by platelet-derived growth factor is the activation of a phosphatidylinositol(4,5)-bisphosphate (3)-hydroxy (PtdIns(4,5)P2 3-OH) kinase. A similar conclusion has been applied to explain the actions of formyl-Met-Leu-Phe on neutrophils, and it may be that receptors that couple through intrinsic tyrosine kinases or through G proteins stimulate the same step in 3-phosphorylated inositol lipid metabolism. The close parallel between these two mechanisms for the activation of PtdIns(4,5)P2 3-OH kinase and those described for the activation of another key signalling enzyme, phospholipase C (ref. 7), focuses attention on the product of the PtdIns(4,5)P2 3-OH kinase, PtdIns(3,4,5)P3, as a possible new second messenger.
Similar articles
-
Both phosphatidylinositol 3-kinase and phosphatidylinositol 4-kinase products are increased by antigen receptor signaling in B cells.J Immunol. 1994 Jan 1;152(1):42-50. J Immunol. 1994. PMID: 8254204
-
Osmotic stress activates phosphatidylinositol-3,5-bisphosphate synthesis.Nature. 1997 Nov 13;390(6656):187-92. doi: 10.1038/36613. Nature. 1997. PMID: 9367158
-
Receptor specificity of growth factor-stimulated synthesis of 3-phosphorylated inositol lipids in Swiss 3T3 cells.J Biol Chem. 1992 Aug 15;267(23):16627-36. J Biol Chem. 1992. PMID: 1322911
-
Phosphoinositide 3-kinase: a new effector in signal transduction?Cell Signal. 1991;3(6):501-13. doi: 10.1016/0898-6568(91)90027-r. Cell Signal. 1991. PMID: 1664737 Review.
-
Lipid kinases: charging PtdIns(4,5)P2 synthesis.Curr Biol. 2010 Feb 23;20(4):R154-7. doi: 10.1016/j.cub.2010.01.016. Curr Biol. 2010. PMID: 20178759 Review.
Cited by
-
Class I PI3K Biology.Curr Top Microbiol Immunol. 2022;436:3-49. doi: 10.1007/978-3-031-06566-8_1. Curr Top Microbiol Immunol. 2022. PMID: 36243838
-
1-Phosphatidylinositol 3-kinase activity is required for insulin-stimulated glucose transport but not for RAS activation in CHO cells.Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7415-9. doi: 10.1073/pnas.91.16.7415. Proc Natl Acad Sci U S A. 1994. PMID: 8052599 Free PMC article.
-
Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85.Mol Cell Biol. 1993 Dec;13(12):7677-88. doi: 10.1128/mcb.13.12.7677-7688.1993. Mol Cell Biol. 1993. PMID: 8246984 Free PMC article.
-
Phosphatidylinositol-3,4,5-triphosphate and cellular signaling: implications for obesity and diabetes.Cell Physiol Biochem. 2015;35(4):1253-75. doi: 10.1159/000373949. Epub 2015 Feb 11. Cell Physiol Biochem. 2015. PMID: 25721445 Free PMC article. Review.
-
AKT in Bone Metastasis of Solid Tumors: A Comprehensive Review.Cancers (Basel). 2021 May 11;13(10):2287. doi: 10.3390/cancers13102287. Cancers (Basel). 2021. PMID: 34064589 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
