Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 May;66(5):2780-91.
doi: 10.1128/JVI.66.5.2780-2791.1992.

Transformation of a continuous rat embryo fibroblast cell line requires three separate domains of simian virus 40 large T antigen

J Zhu  1 , P W Rice, L Gorsch, M Abate, C N Cole
Affiliations

Transformation of a continuous rat embryo fibroblast cell line requires three separate domains of simian virus 40 large T antigen

J Zhu et al. J Virol. 1992 May.

Abstract

Mouse C3H 10T1/2 cells and the established rat embryo fibroblast cell line REF-52 are two cell lines widely used in studies of viral transformation. Studies have shown that transformation of 10T1/2 cells requires only the amino-terminal 121 amino acids of simian virus 40 (SV40) large T antigen, while transformation of REF-52 cells requires considerably more of large T antigen, extending from near the N terminus to beyond residue 600. The ability of a large set of linker insertion, small deletion, and point mutants of SV40 T antigen to transform these two cell lines and to bind p105Rb was determined. Transformation of 10T1/2 cells was greatly reduced by mutations within the first exon of the gene for large T antigen but was only modestly affected by mutations affecting the p105Rb binding site or the p53 binding region. All mutants defective for transformation of 10T1/2 cells were also defective for transformation of REF-52 cells. In addition, mutants whose T antigens had alterations in the Rb binding site showed a substantial reduction in transformation of REF-52 cells, and the degree of this reduction could be correlated with the ability of the mutant T antigens to bind p105Rb. There was a tight correlation between the ability of mutants to transform REF-52 cells and the ability of their T antigens to bind p53. These results demonstrate that multiple regions of large T antigen are required for full transformation by SV40.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1989 Dec;63(12):5459-63 - PubMed
    1. Mol Cell Biol. 1984 Jun;4(6):1125-33 - PubMed
    1. Virology. 1988 Jan;162(1):76-89 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1519-23 - PubMed
    1. J Virol. 1988 Jun;62(6):1999-2006 - PubMed

Publication types