Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 Sep;69(9):5707-10.
doi: 10.1128/AEM.69.9.5707-5710.2003.

Survival of F-specific RNA coliphage, feline calicivirus, and Escherichia coli in water: a comparative study

Paul B Allwood  1 , Yashpal S Malik, Craig W Hedberg, Sagar M Goyal
Affiliations
Comparative Study

Survival of F-specific RNA coliphage, feline calicivirus, and Escherichia coli in water: a comparative study

Paul B Allwood et al. Appl Environ Microbiol. 2003 Sep.

Abstract

The relationship between the survival of enteric viral pathogens and their indicators (coliform bacteria and coliphages) is not well understood. We compared the survival rates of feline calicivirus (FCV), Escherichia coli, and a male-specific RNA coliphage MS2 at 4, 25, and 37 degrees C for up to 28 days in dechlorinated water. The survival rates of E. coli and FCV, a surrogate of noroviruses (NV), had a high degree of correlation at 4 and 25 degrees C, while MS2 phage survived significantly longer (P < 0.05) at these two temperatures. At 37 degrees C, the survival rates for all three organisms were highly correlated. Decimal reduction values indicating the number of days needed for 90% reduction in titer (D values) decreased for all three organisms as storage temperatures increased. FCV had the shortest D value among all three organisms at all temperatures investigated. These findings indicate that F-specific RNA phages may be useful indicators of NV in the environment.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Titers of FCV (▴), F-specific phage MS2 (•), and E. coli (▪) in dechlorinated water stored at 4, 25, or 37°C. Samples were taken at 0, 1, 2, 3, 7, 14, 21, and 28 days. The data points represent the means of the percent change in titers observed over three experimental runs.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abad, F. X., R. M. Pinto, and A. Bosch. 1994. Survival of enteric viruses on environmental fomites. Appl. Environ. Microbiol. 60:3704-3710. - PMC - PubMed
    1. Ando, T., J. S. Noel, and R. L. Fankhauser. 2000. Genetic classification of “Norwalk-like viruses.” J. Infect. Dis. 181:S336-S348. - PubMed
    1. Barrett, E. C., M. D. Sobsey, C. H. House, and K. D. White. 2001. Microbial indicator removal in onsite constructed wetlands for wastewater treatment in the southeastern U.S. Water Sci. Technol. 44:177-182. - PubMed
    1. Beuret, C., D. Kohler, A. Baumgartner, and T. M. Luthi. 2002. Norwalk-like virus sequences in mineral waters: one-year monitoring of three brands. Appl. Environ. Microbiol. 68:1925-1931. - PMC - PubMed
    1. Bidawid, S., N. Malik, O. Adgburin, S. A. Sattar, and J. M. Farber. 2003. A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus. J. Virol. Methods. 107:163-167. - PubMed

Publication types

MeSH terms

LinkOut - more resources