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Comparative Study
. 2003 Sep;22(9):558-60.
doi: 10.1007/s10096-003-0989-0. Epub 2003 Aug 21.

Rapid detection of pathogenic fungi from clinical specimens using LightCycler real-time fluorescence PCR

A Imhof  1 , C Schaer, G Schoedon, D J Schaer, R B Walter, A Schaffner, M Schneemann
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Free article
Comparative Study

Rapid detection of pathogenic fungi from clinical specimens using LightCycler real-time fluorescence PCR

A Imhof et al. Eur J Clin Microbiol Infect Dis. 2003 Sep.
Free article

Abstract

In the study presented here a LightCycler real-time PCR system was used for the diagnosis of fungal infections from clinical tissue samples. Nine specimens were investigated from six patients with suspected or proven invasive fungal infections. Seven of nine samples were positive in a broad-range fungal PCR assay. In four samples, Aspergillus fumigatus was detected both by a species-specific hybridization assay as well as by sequencing of amplification products. In addition, the broad-range fungal PCR assay and PCR sequencing detected and identified, respectively, the following organisms in the specimens noted: Candida albicans in a culture-negative liver biopsy, Histoplasma capsulatum in a bone marrow sample, and Conidiobolus coronatus in a facial soft tissue specimen. Real-time PCR is a promising tool for the diagnosis of invasive fungal infections in human tissue samples and offers some advantages over culture methods, such as rapid analysis and increased sensitivity.

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