[Analysis of PKC targeting mechanism using PKC fused with fluorescent proteins]
- PMID: 12835536
- DOI: 10.1254/fpj.121.421
[Analysis of PKC targeting mechanism using PKC fused with fluorescent proteins]
Abstract
Protein kinase C (PKC) is a family, which consists of at least ten subtypes. To elucidate subtype-specific functions of PKC, we have developed the methods to observe PKC translocation in real time and in the living state using PKC fused with fluorescent proteins, including GFP and DsRed. Based on the live imaging of PKC translocation, we have demonstrated that PKC showed stimulus- and subtype-specific translocation, which can recognize its specific substrate and induce its specific cellular response (PKC targeting). These findings suggest that PKC targeting is the molecular basis underlying the diversity of PKC functions. Live imaging of PKC translocation has been proved to be a beneficial tool for understanding not only PKC functions, but also PKC-mediated signal transduction pathways. We have further analyzed PKC functions in the central nervous system using transgenic mice, which can express PKC-GFP in a brain-region-specific manner.
Similar articles
-
Decoding of short-lived Ca2+ influx signals into long term substrate phosphorylation through activation of two distinct classes of protein kinase C.J Biol Chem. 2003 Mar 14;278(11):9896-904. doi: 10.1074/jbc.M210653200. Epub 2003 Jan 3. J Biol Chem. 2003. PMID: 12514176
-
Subtype-specific translocation of the delta subtype of protein kinase C and its activation by tyrosine phosphorylation induced by ceramide in HeLa cells.Mol Cell Biol. 2001 Mar;21(5):1769-83. doi: 10.1128/MCB.21.5.1769-1783.2001. Mol Cell Biol. 2001. PMID: 11238914 Free PMC article.
-
Myristoylated alanine-rich C-kinase substrate is phosphorylated and translocated by a phorbol ester-insensitive and calcium-independent protein kinase C isoform in C6 glioma cell membranes.Biochim Biophys Acta. 1999 Jan 11;1448(3):439-49. doi: 10.1016/s0167-4889(98)00161-x. Biochim Biophys Acta. 1999. PMID: 9990296
-
Subspecies-specific targeting mechanism of protein kinase C.Jpn J Pharmacol. 1998 Dec;78(4):411-7. doi: 10.1254/jjp.78.411. Jpn J Pharmacol. 1998. PMID: 9920197 Review.
-
[Analysis of molecular mechanism regulating spatio-temporal localization and activity of protein kinase C and diacylglycerol kinase using live imaging].Nihon Yakurigaku Zasshi. 2004 Mar;123(3):189-96. doi: 10.1254/fpj.123.189. Nihon Yakurigaku Zasshi. 2004. PMID: 14993731 Review. Japanese.
Cited by
-
Evolving mechanisms of vascular smooth muscle contraction highlight key targets in vascular disease.Biochem Pharmacol. 2018 Jul;153:91-122. doi: 10.1016/j.bcp.2018.02.012. Epub 2018 Feb 13. Biochem Pharmacol. 2018. PMID: 29452094 Free PMC article. Review.
