Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Jun;41(6):2492-7.
doi: 10.1128/JCM.41.6.2492-2497.2003.

Molecular analysis of human herpesvirus 8 by using single nucleotide polymorphisms in open reading frame 26

Tokiomi Endo  1 , Toshiyuki Miura, Tomohiko Koibuchi, Hitomi Nakamura, Takashi Takahashi, Takashi Odawara, Mieko Goto, Atsushi Ajisawa, Aikichi Iwamoto, Tetsuya Nakamura
Affiliations

Molecular analysis of human herpesvirus 8 by using single nucleotide polymorphisms in open reading frame 26

Tokiomi Endo et al. J Clin Microbiol. 2003 Jun.

Abstract

Human herpesvirus 8 (HHV-8) can be classified into distinct subtypes on the basis of sequence polymorphisms in several open reading frames (ORFs). We analyzed the subtypes of HHV-8 in 59 human immunodeficiency virus-infected Japanese patients by using polymorphisms in ORF26 and found that over two-thirds of the HHV-8 isolates fell into major subtype A. We also found that single nucleotide polymorphisms (SNPs) at nucleotide positions 1032 (C-to-A substitution) and 1055 (G-to-T substitution) in HHV-8 ORF26 were correlated with increased susceptibility to Kaposi's sarcoma, compared to the results obtained with HHV-8 with wild-type nucleotides at these positions (P = 0.0106). This observation suggests that molecular heterogeneity of the HHV-8 genome affects the biological properties of HHV-8, resulting in different clinical phenotypes of HHV-8 infection. Since sensitive PCR of ORF26 allowed us to analyze the SNPs by using peripheral blood from HHV-8-infected patients, the ORF26 SNPs will be a potent tool for investigating the pathogenesis of HHV-8 infection.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Phylogenetic analysis of eight ORF26 SNP types. The sequences of eight types of ORF26 SNPs were aligned and analyzed by CLUSTAL W to produce a phylogenetic tree. The eight SNP types were divided into three groups: group I, types 1 and 2; group II, types 4, 5, and 6; and group III, types 3, 7, and 8.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Beral, V., T. A. Peterman, R. C. Berkelman, and H. W. Jaffe. 1990. Kaposi's sarcoma among persons with AIDS: a sexually transmitted infection? Lancet 335:123-128. - PubMed
    1. Boralevi, F., B. Masquelier, M. Denayrolles, M. Dupon, J. Pellegrin, J. Ragnaud, and H. J. A. Fleury. 1998. Study of human herpesvirus 8 (HHV-8) variants from Kaposi's sarcoma in France: is HHV-8 subtype a responsible for more aggressive tumors? J. Infect. Dis. 178:1546-1547. - PubMed
    1. Boshoff, C., and Y. Chang. 2001. Kaposi's sarcoma-associated herpesvirus: a new DNA tumor virus. Annu. Rev. Med. 52:453-470. - PubMed
    1. Chang, Y., E. Cesarman, M. S. Pession, F. Lee, J. Culpepper, D. M. Knowles, and P. S. Moor. 1994. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science 266:1865-1869. - PubMed
    1. Ciufo, A. M., D. J. Alcendor, X. Wan, J. Nicholas, P. J. Browning, P. L. Rady, S. K. Tyring, J. M. Orenstein, C. S. Rabkin, I.-J. Su, K. F. Powell, M. Croxson, K. E. Foreman, B. J. Nickoloff, S. Alkan, and G. S. Hayward. 1999. High-level variability in the open reading frame K1 membrane protein gene at the left end of the Kaposi's sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. J. Virol. 73:4156-4170. - PMC - PubMed

Publication types