Comparison of the changes in global gene expression of Escherichia coli induced by four bactericidal agents
- PMID: 12736533
- DOI: 10.1159/000069981
Comparison of the changes in global gene expression of Escherichia coli induced by four bactericidal agents
Abstract
DNA microarrays provide a global view of the physiological state of the cell by parallel analysis of the expression levels of all the genes in an organism. The effects of four bactericidal agents on the expression pattern of Escherichia coli MG1655 were assessed. Compounds were chosen on the basis of their different mechanisms of action and included inhibitors of DNA replication and recombination, translation, transcription and cell wall biosynthesis. The addition of rifampin resulted in increased expression of the target, rpoB, as well as several genes involved in nucleotide salvage and purine biosynthesis. The addition of ampicillin resulted in overall changes in gene expression that showed some similarity to changes induced by rifampin. The addition of the antibiotics kanamycin or norfloxacin resulted in the induction of unique gene expression signatures: a heat shock response to kanamycin and an SOS response to norfloxacin. Several genes of unknown function showed expression profiles similar to the genes associated with the SOS or the heat shock response. Thus, these profiles define families of genes with similar expression phenotypes that can be tested for related function.
Copyright 2003 S. Karger AG, Basel
Similar articles
-
A common mechanism of cellular death induced by bactericidal antibiotics.Cell. 2007 Sep 7;130(5):797-810. doi: 10.1016/j.cell.2007.06.049. Cell. 2007. PMID: 17803904
-
On the road to bacterial cell death.Cell. 2007 Sep 7;130(5):781-3. doi: 10.1016/j.cell.2007.08.023. Cell. 2007. PMID: 17803902
-
Bactericidal Antibiotics Induce Toxic Metabolic Perturbations that Lead to Cellular Damage.Cell Rep. 2015 Nov 3;13(5):968-80. doi: 10.1016/j.celrep.2015.09.059. Epub 2015 Oct 22. Cell Rep. 2015. PMID: 26565910 Free PMC article.
-
Antimicrobial Susceptibility and SOS-Dependent Increase in Mutation Frequency Are Impacted by Escherichia coli Topoisomerase I C-Terminal Point Mutation.Antimicrob Agents Chemother. 2015 Oct;59(10):6195-202. doi: 10.1128/AAC.00855-15. Epub 2015 Jul 27. Antimicrob Agents Chemother. 2015. PMID: 26248366 Free PMC article.
-
Global regulators of transcription in Escherichia coli: mechanisms of action and methods for study.Adv Appl Microbiol. 2008;65:93-113. doi: 10.1016/S0065-2164(08)00604-7. Adv Appl Microbiol. 2008. PMID: 19026863 Review. No abstract available.
Cited by
-
Genome-wide identification of Kanamycin B binding RNA in Escherichia coli.BMC Genomics. 2023 Mar 16;24(1):120. doi: 10.1186/s12864-023-09234-3. BMC Genomics. 2023. PMID: 36927548 Free PMC article.
-
Metabolic engineering in the -omics era: elucidating and modulating regulatory networks.Microbiol Mol Biol Rev. 2005 Jun;69(2):197-216. doi: 10.1128/MMBR.69.2.197-216.2005. Microbiol Mol Biol Rev. 2005. PMID: 15944454 Free PMC article. Review.
-
Epigenetic inheritance based evolution of antibiotic resistance in bacteria.BMC Evol Biol. 2008 Feb 18;8:52. doi: 10.1186/1471-2148-8-52. BMC Evol Biol. 2008. PMID: 18282299 Free PMC article.
-
C Group-Mediated Antibiotic Stress Mimics the Cold Shock Response.Curr Microbiol. 2021 Sep;78(9):3372-3380. doi: 10.1007/s00284-021-02613-7. Epub 2021 Jul 20. Curr Microbiol. 2021. PMID: 34283283 Review.
-
Medium plays a role in determining expression of acrB, marA, and soxS in Escherichia coli.Antimicrob Agents Chemother. 2006 Mar;50(3):1071-4. doi: 10.1128/AAC.50.3.1071-1074.2006. Antimicrob Agents Chemother. 2006. PMID: 16495271 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
