doi: 10.1016/0005-2736(76)90391-6.
Isolation of membrane glycoproteins by affinity chromatography in the presence of detergents
- PMID: 1268207
- DOI: 10.1016/0005-2736(76)90391-6
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Isolation of membrane glycoproteins by affinity chromatography in the presence of detergents
Biochim Biophys Acta.
.
Abstract
Wheat germ agglutinin has been used in a one-step preparative method to isolate the major sialoglycoprotein (glycophorin A) from the human erythrocyte membrane. The conditions for isolation and purification of the sialoglycopeptide included low concentration of sodium dodecyl sulfate in the presence of relatively high salt concentration. This medium caused complete solubilization of the membrane but still allowed almost quantitative binding of the sialoglycopeptide to wheat germ agglutinin-Sepharose. The eluted protein from such affinity systems was found to be chemically comparable to glycophorin A, as prepared by other procedures.
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