Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Dec;34(6):507-16.
doi: 10.1023/a:1022578428026.

The key role of the energized state of Saccharomyces cerevisiae mitochondria in modulations of the outer membrane channels by the intermembrane space proteins

Olgierd Stobienia  1 , Sylwia Wróblewska, Nina Antos, Malgorzata Budzińska, Hanna Kmita
Affiliations

The key role of the energized state of Saccharomyces cerevisiae mitochondria in modulations of the outer membrane channels by the intermembrane space proteins

Olgierd Stobienia et al. J Bioenerg Biomembr. 2002 Dec.

Abstract

Mitochondria of the yeast Saccharomyces cerevisiae constitute a perfect model to study the outer membrane channel modulation as besides the TOM complex channel they contain only a single isoform of the VDAC channel and it is possible to obtain viable mutants devoid of the channel. Here, we report that the fraction of the intermembrane space isolated from wild type and the VDAC channel-depleted yeast mitochondria, except of the well-known VDAC channel modulator activity, displays also the TOM complex channel modulating activity as measured in the reconstituted system and with intact mitochondria. The important factor influencing the action of both modulating activities is the energized state of mitochondria. Moreover, the presence of the VDAC channel itself seems to be crucial to properties of the intermembrane space protein(s) able to modulate the outer membrane channels because in the case of intact mitochondria quantitative differences are observed between modulating capabilities of the fractions isolated from wild type and mutant mitochondria.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Am J Physiol. 1991 Feb;260(2 Pt 1):C371-4 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Biochimie. 2002 Feb-Mar;84(2-3):187-93 - PubMed
    1. Mol Cell Biol. 1998 Sep;18(9):5256-62 - PubMed
    1. J Biol Chem. 1994 Oct 21;269(42):26402-10 - PubMed

MeSH terms

LinkOut - more resources