Monoallelic expression of the murine gene encoding Toll-like receptor 4
- PMID: 12665857
- DOI: 10.1038/ni917
Monoallelic expression of the murine gene encoding Toll-like receptor 4
Abstract
Defects in the gene encoding Toll-like receptor 4 (Tlr4) result in impaired responses to lipopolysaccharide (LPS), rendering mice sensitive to infections by Gram-negative bacteria. C3H/HeJ mice have a codominant allele with a mutation in Tlr4, which results in an intermediate response to LPS in F1 mice from crosses of responder and C3H/HeJ mice. Here we show that this intermediate response to LPS is due to monoallelic expression of Tlr4. Allele usage is maintained during clonal expansion, a situation that resembles allelic exclusion. In contrast, Tlr4 is deleted on the recessive C57BL/10ScCr allele and all cells from F1 mice from crosses of responder and C57BL/10ScCr mice express TLR4 protein. Thus, Tlr4 is an autosomal gene whose expression is regulated similarly to that of genes on the X chromosome.
Similar articles
-
Toll-like receptor 4 is not required for the full maturation of dendritic cells or for the degradation of Gram-negative bacteria.Eur J Immunol. 2002 Oct;32(10):2800-6. doi: 10.1002/1521-4141(2002010)32:10<2800::AID-IMMU2800>3.0.CO;2-5. Eur J Immunol. 2002. PMID: 12355432
-
Cooperation between toll-like receptor 2 and 4 in the brain of mice challenged with cell wall components derived from gram-negative and gram-positive bacteria.Eur J Immunol. 2003 Apr;33(4):1127-38. doi: 10.1002/eji.200323821. Eur J Immunol. 2003. PMID: 12672079
-
Cutting edge: functional characterization of the effect of the C3H/HeJ defect in mice that lack an Lpsn gene: in vivo evidence for a dominant negative mutation.J Immunol. 1999 May 15;162(10):5666-70. J Immunol. 1999. PMID: 10229796
-
Positional cloning of Lps, and the general role of toll-like receptors in the innate immune response.Eur Cytokine Netw. 2000 Jun;11(2):143-52. Eur Cytokine Netw. 2000. PMID: 10903793 Review.
-
Lps: another piece in the puzzle.J Endotoxin Res. 2000;6(4):295-300; discussion 301-2. J Endotoxin Res. 2000. PMID: 11521048 Review. No abstract available.
Cited by
-
Risk alleles of genes with monoallelic expression are enriched in gain-of-function variants and depleted in loss-of-function variants for neurodevelopmental disorders.Mol Psychiatry. 2017 Dec;22(12):1785-1794. doi: 10.1038/mp.2017.13. Epub 2017 Mar 7. Mol Psychiatry. 2017. PMID: 28265118 Free PMC article.
-
RNA sequencing-based screen for reactivation of silenced alleles of autosomal genes.G3 (Bethesda). 2022 Feb 4;12(2):jkab428. doi: 10.1093/g3journal/jkab428. G3 (Bethesda). 2022. PMID: 35100361 Free PMC article.
-
Dynamin 2-dependent endocytosis is required for sustained S1PR1 signaling.J Exp Med. 2014 Apr 7;211(4):685-700. doi: 10.1084/jem.20131343. Epub 2014 Mar 17. J Exp Med. 2014. PMID: 24638168 Free PMC article.
-
The Many Alternative Faces of Macrophage Activation.Front Immunol. 2015 Jul 22;6:370. doi: 10.3389/fimmu.2015.00370. eCollection 2015. Front Immunol. 2015. PMID: 26257737 Free PMC article. Review.
-
Monoallelic expression of Krt12 gene during corneal-type epithelium differentiation of limbal stem cells.Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4562-8. doi: 10.1167/iovs.10-5331. Epub 2010 Apr 14. Invest Ophthalmol Vis Sci. 2010. PMID: 20393120 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
