Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Feb 20;338(1):29-32.
doi: 10.1016/s0304-3940(02)01364-2.

Temporal regulation of CD81 following retinal injury in the rat

Bong Keun Song  1 , Grace R Geisert, Felix Vázquez-Chona, Eldon E Geisert Jr
Affiliations

Temporal regulation of CD81 following retinal injury in the rat

Bong Keun Song et al. Neurosci Lett. .

Abstract

Following retinal injury, glial cells within the retina undergo a response that is characterized by the proliferation of astrocytes, Müller cells, and retinal pigment epithelial cells. CD81, a small membrane protein known to be involved in cell proliferation, is up-regulated after injury. This study focuses on the temporal regulation of CD81, relating the expression of this protein to glial fibrillary acidic protein (GFAP), the classic marker of gliosis. CD81 levels were elevated at 7 days after injury and remained elevated at 30 days after injury; GFAP was increased at 7 days and continued to increase until 30 days post injury. This association of CD81 with glial reactivity may provide a clue to the regulation of the proliferative response following retinal injury.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The response of the retina to injury is illustrated in four photomicrographs taken 7 days after placement of a retinal tear. Two basic responses to injury can be seen. (A) The site of the induced tear (arrow) is filled with GFAP-positive cells (Müller glia or astrocytes). (B) In an adjacent section, the glial scar demonstrates a substantial increase in CD81 immunostaining. At other sites of the retinal tear, the cells filling the site of injury are GFAP-negative (C) but nonetheless show high levels of CD81 immunoreactivity (D). Note the cellular tissues (white arrows) and erythrocytes (white arrow heads) that extend out into the vitreal space. All of the photomicrographs at the same magnification; the scale bar in D = 100 μm.
Fig. 2
Fig. 2
The levels of CD81, GFAP and cytokeratin were quantified using immunoblot methods at 7 and 30 days following injury. Note that the levels of all three proteins, expressed in relative absorbance units, increased after injury. CD81 expression was significantly up-regulated at 7 days after injury and remained elevated at 30 days. The levels of GFAP were up-regulated approximately 1.5- and 2-fold at 7 days and 30 days, respectively. Cytokeratin was increased 2.5-fold at 7 days after injury and 3-fold at 30 days after injury. Asterisks denote significant differences from the normal: (Mann–Whitney U test, *P < 0.05, **P < 0.01).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Berditchevski F, Tolias KF, Wong K, Carpenter CL, Hemler ME. A novel link between integrins, transmembrane-4 superfamily proteins (CD63 and CD81), and phosphatidylinositol 4-kinase. J Biol Chem. 1997;272:2595–2598. - PubMed
    1. Boucheix C, Rubinstein E. Tetraspanins. Cell Mol Life Sci. 2001;58:1189–1205. - PMC - PubMed
    1. Bignami A, Dahl D. The radial glia of Müller in the rat retina and their response to injury: an immunofluorescence study with antibodies to the glial fibrillary acidic (GFA) protein. Exp Eye Res. 1979;28:63–69. - PubMed
    1. Clarke K, Geisert EE., Jr The target of the antiproliferative antibody (TAPA) in the normal and injured rat retina. Mol Vis. 1998;4:3. - PubMed
    1. Dijkstra S, Geisert EE, Jr, Gispen WH, Bar PR, Joosten EAJ. Up-regulation of CD81 (target of the antiproliferative antibody; TAPA) by reactive microglia and astrocytes after spinal cord injury in the rat. J Comp Neurol. 2000;428:266–277. - PubMed

Publication types

LinkOut - more resources