RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells
- PMID: 12168660
- DOI: 10.1016/S0736-0266(02)00006-2
RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells
Abstract
Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via its N-terminal domain and to the transcription and translation factor Y-box binding protein-1 (YB-1) through its C-terminal domain. The liposarcoma fusion protein TLS/CHOP retains the ability to bind RNA Pol II but lacks the ability to recruit YB-1 due to replacement of the C-terminal domain of TLS by CHOP. In an in vivo splicing assay, YB-1 promotes splicing of adenovirus EIA pre-mRNA predominantly to the 13S isoform. The oncogenic TLS/CHOP fusion protein inhibits this splicing function of YB-1 in a dominant negative manner. When considered in conjunction with studies on other sarcoma fusion proteins, these data suggest that aberrant RNA splicing may be a common feature of human sarcomas.
Similar articles
-
Oncogenic TLS/ERG and EWS/Fli-1 fusion proteins inhibit RNA splicing mediated by YB-1 protein.Cancer Res. 2001 May 1;61(9):3586-90. Cancer Res. 2001. PMID: 11325824
-
Chimeric TLS/FUS-CHOP gene expression and the heterogeneity of its junction in human myxoid and round cell liposarcoma.Am J Pathol. 1995 Nov;147(5):1221-7. Am J Pathol. 1995. PMID: 7485386 Free PMC article.
-
Characteristics of genomic breakpoints in TLS-CHOP translocations in liposarcomas suggest the involvement of Translin and topoisomerase II in the process of translocation.Oncogene. 1999 Jan 21;18(3):721-9. doi: 10.1038/sj.onc.1202364. Oncogene. 1999. PMID: 9989822
-
TLS-CHOP and the role of RNA-binding proteins in oncogenic transformation.Curr Top Microbiol Immunol. 1997;220:131-42. doi: 10.1007/978-3-642-60479-9_8. Curr Top Microbiol Immunol. 1997. PMID: 9103679 Review. No abstract available.
-
TLS, EWS and TAF15: a model for transcriptional integration of gene expression.Brief Funct Genomic Proteomic. 2006 Mar;5(1):8-14. doi: 10.1093/bfgp/ell015. Epub 2006 Feb 23. Brief Funct Genomic Proteomic. 2006. PMID: 16769671 Review.
Cited by
-
NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.Nucleic Acids Res. 2014 Mar;42(5):3177-93. doi: 10.1093/nar/gkt1311. Epub 2013 Dec 17. Nucleic Acids Res. 2014. PMID: 24353314 Free PMC article.
-
Exosomal YB-1 facilitates ovarian restoration by MALAT1/miR-211-5p/FOXO3 axis.Cell Biol Toxicol. 2024 May 3;40(1):29. doi: 10.1007/s10565-024-09871-8. Cell Biol Toxicol. 2024. PMID: 38700571 Free PMC article.
-
TDP-43 and FUS/TLS: emerging roles in RNA processing and neurodegeneration.Hum Mol Genet. 2010 Apr 15;19(R1):R46-64. doi: 10.1093/hmg/ddq137. Epub 2010 Apr 15. Hum Mol Genet. 2010. PMID: 20400460 Free PMC article. Review.
-
RNA Dysregulation in Amyotrophic Lateral Sclerosis.Front Genet. 2019 Jan 22;9:712. doi: 10.3389/fgene.2018.00712. eCollection 2018. Front Genet. 2019. PMID: 30723494 Free PMC article. Review.
-
Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation.Histochem Cell Biol. 2024 May;161(5):435-444. doi: 10.1007/s00418-024-02272-2. Epub 2024 Feb 23. Histochem Cell Biol. 2024. PMID: 38396247
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
