Strategies for microsatellite isolation: a review
- PMID: 11903900
- DOI: 10.1046/j.0962-1083.2001.01418.x
Strategies for microsatellite isolation: a review
Abstract
In the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.
Similar articles
-
[Strategies for development of SSR molecular markers].Yi Chuan. 2004 Sep;26(5):763-8. Yi Chuan. 2004. PMID: 15640099 Review. Chinese.
-
Single locus microsatellites isolated using 5' anchored PCR.Nucleic Acids Res. 1996 Nov 1;24(21):4369-71. doi: 10.1093/nar/24.21.4369. Nucleic Acids Res. 1996. PMID: 8932400 Free PMC article.
-
Microsatellite DNA capture from enriched libraries.Methods Mol Biol. 2013;1006:67-87. doi: 10.1007/978-1-62703-389-3_5. Methods Mol Biol. 2013. PMID: 23546784
-
Design and implementation of degenerate microsatellite primers for the mammalian clade.PLoS One. 2011;6(12):e29582. doi: 10.1371/journal.pone.0029582. Epub 2011 Dec 27. PLoS One. 2011. PMID: 22216321 Free PMC article.
-
Evolutionary dynamics of microsatellite DNA.Chromosoma. 2000 Sep;109(6):365-71. doi: 10.1007/s004120000089. Chromosoma. 2000. PMID: 11072791 Review.
Cited by
-
Development of chromosome-specific markers with high polymorphism for allotetraploid cotton based on genome-wide characterization of simple sequence repeats in diploid cottons (Gossypium arboreum L. and Gossypium raimondii Ulbrich).BMC Genomics. 2015 Feb 6;16(1):55. doi: 10.1186/s12864-015-1265-2. BMC Genomics. 2015. PMID: 25652321 Free PMC article.
-
Population genetic variation characterization of the boreal tree Acer ginnala in Northern China.Sci Rep. 2020 Aug 11;10(1):13515. doi: 10.1038/s41598-020-70444-w. Sci Rep. 2020. PMID: 32782277 Free PMC article.
-
Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives.Genet Res Int. 2015;2015:431487. doi: 10.1155/2015/431487. Epub 2015 Mar 19. Genet Res Int. 2015. PMID: 25874132 Free PMC article. Review.
-
Development of novel microsatellite markers in the Omei treefrog (Rhacophorus omeimontis).Int J Mol Sci. 2012;13(1):552-557. doi: 10.3390/ijms13010552. Epub 2012 Jan 5. Int J Mol Sci. 2012. PMID: 22312271 Free PMC article.
-
Turtles in Malaysia: A Review of Conservation Status and a Call for Research.Animals (Basel). 2022 Aug 25;12(17):2184. doi: 10.3390/ani12172184. Animals (Basel). 2022. PMID: 36077905 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
